DNA-directed three-dimensional protein organization

Chuan Zhang, Cheng Tian, Fei Guo, Zheng Liu, Wen Jiang, Chengde Mao

Research output: Contribution to journalArticlepeer-review

94 Scopus citations

Abstract

All bound together: Self-assembled symmetric DNA polyhedra were used to organize proteins in 3D space. Biotin moieties were incorporated into the self-assembled symmetric DNA polyhedra. Upon incubation with streptavidin (STV) protein, an STV protein became bound to each polyhedral face, thus resulting in well-structured DNA polyhedra/STV complexes (see picture, TET=tetrahedron). This strategy was also applied to different 3D DNA nanostructures and different proteins.

Original languageEnglish (US)
Pages (from-to)3382-3385
Number of pages4
JournalAngewandte Chemie - International Edition
Volume51
Issue number14
DOIs
StatePublished - Apr 2 2012

All Science Journal Classification (ASJC) codes

  • Catalysis
  • General Chemistry

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