DNA-directed three-dimensional protein organization

All bound together: Self-assembled symmetric DNA polyhedra were used to organize proteins in 3D space. Biotin moieties were incorporated into the self-assembled symmetric DNA polyhedra. Upon incubation with streptavidin (STV) protein, an STV protein became bound to each polyhedral face, thus resulting in well-structured DNA polyhedra/STV complexes (see picture, TET=tetrahedron). This strategy was also applied to different 3D DNA nanostructures and different proteins.