DNA separations from nanovials

Paula Beyer Hietpas; Andrew G. Ewing

doi:10.1081/JLC-100101432

DNA separations from nanovials

Paula Beyer Hietpas, Andrew G. Ewing

Chemistry

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Integration of 1-nL sampling vials with a capillary sample introduction ultrathin slab gel electrophoresis system is described here. The low-volume sampling capabilities of capillary electrophoresis (CE) along with the small structural size make it an ideal sampling technique for these vials. A DNA restriction enzyme digest has been sampled and separated from these vials. A comparison of the nanovial DNA separation and a separation from a larger vial are virtually identical.

Original language	English (US)
Pages (from-to)	15-24
Number of pages	10
Journal	Journal of Liquid Chromatography and Related Technologies
Volume	23
Issue number	1
DOIs	https://doi.org/10.1081/JLC-100101432
State	Published - 2000

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Pharmaceutical Science
Clinical Biochemistry

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10.1081/JLC-100101432

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abstract = "Integration of 1-nL sampling vials with a capillary sample introduction ultrathin slab gel electrophoresis system is described here. The low-volume sampling capabilities of capillary electrophoresis (CE) along with the small structural size make it an ideal sampling technique for these vials. A DNA restriction enzyme digest has been sampled and separated from these vials. A comparison of the nanovial DNA separation and a separation from a larger vial are virtually identical.",

author = "Hietpas, {Paula Beyer} and Ewing, {Andrew G.}",

note = "Funding Information: Support from the National Institutes of Health is gratefully acknowledged. We thank Beth Hall and Leeanne Kiem at the Cornell Nanofabrication Facility for their assistance in the nanovial preparations.",

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AU - Hietpas, Paula Beyer

AU - Ewing, Andrew G.

N1 - Funding Information: Support from the National Institutes of Health is gratefully acknowledged. We thank Beth Hall and Leeanne Kiem at the Cornell Nanofabrication Facility for their assistance in the nanovial preparations.

PY - 2000

Y1 - 2000

N2 - Integration of 1-nL sampling vials with a capillary sample introduction ultrathin slab gel electrophoresis system is described here. The low-volume sampling capabilities of capillary electrophoresis (CE) along with the small structural size make it an ideal sampling technique for these vials. A DNA restriction enzyme digest has been sampled and separated from these vials. A comparison of the nanovial DNA separation and a separation from a larger vial are virtually identical.

AB - Integration of 1-nL sampling vials with a capillary sample introduction ultrathin slab gel electrophoresis system is described here. The low-volume sampling capabilities of capillary electrophoresis (CE) along with the small structural size make it an ideal sampling technique for these vials. A DNA restriction enzyme digest has been sampled and separated from these vials. A comparison of the nanovial DNA separation and a separation from a larger vial are virtually identical.

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DNA separations from nanovials

Abstract

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