DNA synthesis in adult rat anterior pituitary glands in organ culture

Andrea Mastro; W. C. Hymer; C. D. Therrien

doi:10.1016/0014-4827(69)90222-5

DNA synthesis in adult rat anterior pituitary glands in organ culture

Andrea Mastro, W. C. Hymer, C. D. Therrien

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Anterior pituitary glands of adult male rats weighing 175-500 g were cultured for periods up to 13 h in Medium 199 containing 2 μc/ml of ³H-thymidine. Extraction of the nucleic acids from pituitary homogenates by conventional biochemical procedures indicated that the ³H-thymidine was incorporated into DNA. In addition, radioautographs showed that the newly synthesized DNA was nuclear and in pituitary parenchymal cells. Labeled mitotic figures were found, and labeled acidophils and basophils were also observed. These results indicate that pituitary cells committed to the synthesis of specific hormones can also synthesize DNA.

Original language	English (US)
Pages (from-to)	407-414
Number of pages	8
Journal	Experimental Cell Research
Volume	54
Issue number	3
DOIs	https://doi.org/10.1016/0014-4827(69)90222-5
State	Published - Mar 1969

All Science Journal Classification (ASJC) codes

Cell Biology

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10.1016/0014-4827(69)90222-5

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title = "DNA synthesis in adult rat anterior pituitary glands in organ culture",

abstract = "Anterior pituitary glands of adult male rats weighing 175-500 g were cultured for periods up to 13 h in Medium 199 containing 2 μc/ml of 3H-thymidine. Extraction of the nucleic acids from pituitary homogenates by conventional biochemical procedures indicated that the 3H-thymidine was incorporated into DNA. In addition, radioautographs showed that the newly synthesized DNA was nuclear and in pituitary parenchymal cells. Labeled mitotic figures were found, and labeled acidophils and basophils were also observed. These results indicate that pituitary cells committed to the synthesis of specific hormones can also synthesize DNA.",

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year = "1969",

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AU - Hymer, W. C.

AU - Therrien, C. D.

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N2 - Anterior pituitary glands of adult male rats weighing 175-500 g were cultured for periods up to 13 h in Medium 199 containing 2 μc/ml of 3H-thymidine. Extraction of the nucleic acids from pituitary homogenates by conventional biochemical procedures indicated that the 3H-thymidine was incorporated into DNA. In addition, radioautographs showed that the newly synthesized DNA was nuclear and in pituitary parenchymal cells. Labeled mitotic figures were found, and labeled acidophils and basophils were also observed. These results indicate that pituitary cells committed to the synthesis of specific hormones can also synthesize DNA.

AB - Anterior pituitary glands of adult male rats weighing 175-500 g were cultured for periods up to 13 h in Medium 199 containing 2 μc/ml of 3H-thymidine. Extraction of the nucleic acids from pituitary homogenates by conventional biochemical procedures indicated that the 3H-thymidine was incorporated into DNA. In addition, radioautographs showed that the newly synthesized DNA was nuclear and in pituitary parenchymal cells. Labeled mitotic figures were found, and labeled acidophils and basophils were also observed. These results indicate that pituitary cells committed to the synthesis of specific hormones can also synthesize DNA.

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DNA synthesis in adult rat anterior pituitary glands in organ culture

Abstract

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