DNA synthesis is not required for the commitment of murine erythroleukemia cells

Robert Levenson; Joseph Kernen; Alberto Mitrani; David Housman

doi:10.1016/0012-1606(80)90064-0

DNA synthesis is not required for the commitment of murine erythroleukemia cells

Robert Levenson
, Joseph Kernen
, Alberto Mitrani
, David Housman

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

We have assessed the relationship between DNA synthesis and the differentiation of MEL cells induced by DMSO. Under conditions where the rate of incorporation of ³H-deoxyadenosine into DNA was inhibited by 99%, the rate at which MEL cells become committed to terminal erythroid differentiation was identical to that of a culture treated with inducer alone. We conclude that commitment of MEL cells does not require concomitant DNA synthesis.

Original language	English (US)
Pages (from-to)	224-230
Number of pages	7
Journal	Developmental biology
Volume	74
Issue number	1
DOIs	https://doi.org/10.1016/0012-1606(80)90064-0
State	Published - Jan 1980

All Science Journal Classification (ASJC) codes

Molecular Biology
Developmental Biology
Cell Biology

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10.1016/0012-1606(80)90064-0

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title = "DNA synthesis is not required for the commitment of murine erythroleukemia cells",

abstract = "We have assessed the relationship between DNA synthesis and the differentiation of MEL cells induced by DMSO. Under conditions where the rate of incorporation of 3H-deoxyadenosine into DNA was inhibited by 99\%, the rate at which MEL cells become committed to terminal erythroid differentiation was identical to that of a culture treated with inducer alone. We conclude that commitment of MEL cells does not require concomitant DNA synthesis.",

author = "Robert Levenson and Joseph Kernen and Alberto Mitrani and David Housman",

note = "Funding Information: Thii study was supportedb y a grant from the NIH (Ca-17575)t o D. Housman and a grant from the National Cancer Institute (CA-14051)t o the Center for Cancer Research (Dr. S. Lnria, principal investigator).R . Levensoni s a fellow of the Leukemia Society of America.",

year = "1980",

month = jan,

doi = "10.1016/0012-1606(80)90064-0",

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pages = "224--230",

journal = "Developmental biology",

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T1 - DNA synthesis is not required for the commitment of murine erythroleukemia cells

AU - Levenson, Robert

AU - Kernen, Joseph

AU - Mitrani, Alberto

AU - Housman, David

N1 - Funding Information: Thii study was supportedb y a grant from the NIH (Ca-17575)t o D. Housman and a grant from the National Cancer Institute (CA-14051)t o the Center for Cancer Research (Dr. S. Lnria, principal investigator).R . Levensoni s a fellow of the Leukemia Society of America.

PY - 1980/1

Y1 - 1980/1

N2 - We have assessed the relationship between DNA synthesis and the differentiation of MEL cells induced by DMSO. Under conditions where the rate of incorporation of 3H-deoxyadenosine into DNA was inhibited by 99%, the rate at which MEL cells become committed to terminal erythroid differentiation was identical to that of a culture treated with inducer alone. We conclude that commitment of MEL cells does not require concomitant DNA synthesis.

AB - We have assessed the relationship between DNA synthesis and the differentiation of MEL cells induced by DMSO. Under conditions where the rate of incorporation of 3H-deoxyadenosine into DNA was inhibited by 99%, the rate at which MEL cells become committed to terminal erythroid differentiation was identical to that of a culture treated with inducer alone. We conclude that commitment of MEL cells does not require concomitant DNA synthesis.

UR - https://www.scopus.com/pages/publications/0018891158

UR - https://www.scopus.com/pages/publications/0018891158#tab=citedBy

U2 - 10.1016/0012-1606(80)90064-0

DO - 10.1016/0012-1606(80)90064-0

M3 - Article

C2 - 6444216

AN - SCOPUS:0018891158

SN - 0012-1606

VL - 74

SP - 224

EP - 230

JO - Developmental biology

JF - Developmental biology

IS - 1

ER -

DNA synthesis is not required for the commitment of murine erythroleukemia cells

Abstract

All Science Journal Classification (ASJC) codes

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