DNA Translocation in Nanometer Thick Silicon Nanopores

Julio A. Rodríguez-Manzo, Matthew Puster, Adrien Nicolaï, Vincent Meunier, Marija Drndić

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

Solid-state nanopores are single-molecule sensors that detect changes in ionic conductance (δG) when individual molecules pass through them. Producing high signal-to-noise ratio for the measurement of molecular structure in applications such as DNA sequencing requires low noise and large δG. The latter is achieved by reducing the nanopore diameter and membrane thickness. While the minimum diameter is limited by the molecule size, the membrane thickness is constrained by material properties. We use molecular dynamics simulations to determine the theoretical thickness limit of amorphous Si membranes to be -1 nm, and we designed an electron-irradiation-based thinning method to reach that limit and drill nanopores in the thinned regions. Double-stranded DNA translocations through these nanopores (down to 1.4 nm in thickness and 2.5 nm in diameter) provide the intrinsic ionic conductance detection limit in Si-based nanopores. In this regime, where the access resistance is comparable to the nanopore resistance, we observe the appearance of two conductance levels during molecule translocation. Considering the overall performance of Si-based nanopores, our work highlights their potential as a leading material for sequencing applications.

Original languageEnglish (US)
Pages (from-to)6555-6564
Number of pages10
JournalACS nano
Volume9
Issue number6
DOIs
StatePublished - Jun 23 2015

All Science Journal Classification (ASJC) codes

  • General Materials Science
  • General Engineering
  • General Physics and Astronomy

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