TY - JOUR
T1 - Dopamine concentration in the cytoplasmic compartment of single neurons determined by capillary electrophoresis
AU - Olefirowicz, Teresa M.
AU - Ewing, Andrew G.
N1 - Funding Information:
The authors would like to thank S. Stranick for technical assistance. This work was supported by grants from the National Institutes of Health (R01 GM37621-03), Beckman Instruments and Shell Development. A.G.E. is the recipient of a Presidential Young Investigator Award (CHE-8657193), an Alfred P. Sloan Research Fellowship and is a Dreyfus Research Scholar.
PY - 1990/9
Y1 - 1990/9
N2 - It has long been a goal of the biochemist to study the chemistry of single cells. This is particularly important in neuroscience, since each cell can maintain a specific function and identity. Investigations into neurotransmitter compartmentalization within the cell body of neurons should lead to an understanding of the role of the cell body in metabolism, uptake and storage of neurotransmitters. Using capillary electrophoresis, it has been possible to separate and detect attomole levels of neurotransmitter in picoliter volumes of cytoplasm withdrawn from single neurons of the pond snail, Planorbis corneus. This work demonstrates, for the first time, the direct determination of the cytoplasmic concentration of dopamine in single, intact neurons.
AB - It has long been a goal of the biochemist to study the chemistry of single cells. This is particularly important in neuroscience, since each cell can maintain a specific function and identity. Investigations into neurotransmitter compartmentalization within the cell body of neurons should lead to an understanding of the role of the cell body in metabolism, uptake and storage of neurotransmitters. Using capillary electrophoresis, it has been possible to separate and detect attomole levels of neurotransmitter in picoliter volumes of cytoplasm withdrawn from single neurons of the pond snail, Planorbis corneus. This work demonstrates, for the first time, the direct determination of the cytoplasmic concentration of dopamine in single, intact neurons.
UR - http://www.scopus.com/inward/record.url?scp=0024995875&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024995875&partnerID=8YFLogxK
U2 - 10.1016/0165-0270(90)90036-F
DO - 10.1016/0165-0270(90)90036-F
M3 - Article
C2 - 2259233
AN - SCOPUS:0024995875
SN - 0165-0270
VL - 34
SP - 11
EP - 15
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 1-3
ER -