Dopamine type 2 receptor expression and function in rodent sensory neurons projecting to the airways

Christian Peiser; Marcello Trevisani; David A. Groneberg; Q. Thai Dinh; Doerthe Lencer; Silvia Amadesi; Barbara Maggiore; Selena Harrison; Pierangelo Geppetti; Axel Fischer

doi:10.1152/ajplung.00222.2004

Dopamine type 2 receptor expression and function in rodent sensory neurons projecting to the airways

Christian Peiser, Marcello Trevisani, David A. Groneberg, Q. Thai Dinh, Doerthe Lencer, Silvia Amadesi, Barbara Maggiore, Selena Harrison, Pierangelo Geppetti, Axel Fischer

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Agonists of the dopamine receptors have been demonstrated to have bronchodilatory properties in pathologically constricted airways. The mechanism by which these agonists induce bronchodilatation is thought to involve airway sensory nerves. In this study, the expression and function of dopamine D ₂ receptor were examined in sensory ganglia supplying the airways; Neuronal dopamine D₂ receptor mRNA expression was demonstrated by single-cell RT-PCR following laser-assisted microdissection. The projection of the neurons to the airways was confirmed by retrograde neuronal labeling. In functional studies, dopamine D₂ receptor agonists (AR-C65116AB and ropinirole) inhibited intraneuronal calcium mobilization in rat capsaicin-sensitive primary sensory neurons and capsaicin-induced plasma extravasation in the rat trachea. Our results provide support to the hypothesis that dopamine D₂ receptor activation inhibits neurogenic inflammation and proinflammatory reflex responses.

Original language	English (US)
Pages (from-to)	L153-L158
Journal	American Journal of Physiology - Lung Cellular and Molecular Physiology
Volume	289
Issue number	1 33-1
DOIs	https://doi.org/10.1152/ajplung.00222.2004
State	Published - Jul 2005

All Science Journal Classification (ASJC) codes

Physiology
Pulmonary and Respiratory Medicine
Physiology (medical)
Cell Biology

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Peiser, C., Trevisani, M., Groneberg, D. A., Thai Dinh, Q., Lencer, D., Amadesi, S., Maggiore, B., Harrison, S., Geppetti, P., & Fischer, A. (2005). Dopamine type 2 receptor expression and function in rodent sensory neurons projecting to the airways. American Journal of Physiology - Lung Cellular and Molecular Physiology, 289(1 33-1), L153-L158. https://doi.org/10.1152/ajplung.00222.2004

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title = "Dopamine type 2 receptor expression and function in rodent sensory neurons projecting to the airways",

abstract = "Agonists of the dopamine receptors have been demonstrated to have bronchodilatory properties in pathologically constricted airways. The mechanism by which these agonists induce bronchodilatation is thought to involve airway sensory nerves. In this study, the expression and function of dopamine D 2 receptor were examined in sensory ganglia supplying the airways; Neuronal dopamine D2 receptor mRNA expression was demonstrated by single-cell RT-PCR following laser-assisted microdissection. The projection of the neurons to the airways was confirmed by retrograde neuronal labeling. In functional studies, dopamine D2 receptor agonists (AR-C65116AB and ropinirole) inhibited intraneuronal calcium mobilization in rat capsaicin-sensitive primary sensory neurons and capsaicin-induced plasma extravasation in the rat trachea. Our results provide support to the hypothesis that dopamine D2 receptor activation inhibits neurogenic inflammation and proinflammatory reflex responses.",

author = "Christian Peiser and Marcello Trevisani and Groneberg, {David A.} and {Thai Dinh}, Q. and Doerthe Lencer and Silvia Amadesi and Barbara Maggiore and Selena Harrison and Pierangelo Geppetti and Axel Fischer",

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Peiser, C, Trevisani, M, Groneberg, DA, Thai Dinh, Q, Lencer, D, Amadesi, S, Maggiore, B, Harrison, S, Geppetti, P & Fischer, A 2005, 'Dopamine type 2 receptor expression and function in rodent sensory neurons projecting to the airways', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 289, no. 1 33-1, pp. L153-L158. https://doi.org/10.1152/ajplung.00222.2004

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AU - Peiser, Christian

AU - Trevisani, Marcello

AU - Groneberg, David A.

AU - Thai Dinh, Q.

AU - Lencer, Doerthe

AU - Amadesi, Silvia

AU - Maggiore, Barbara

AU - Harrison, Selena

AU - Geppetti, Pierangelo

AU - Fischer, Axel

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AB - Agonists of the dopamine receptors have been demonstrated to have bronchodilatory properties in pathologically constricted airways. The mechanism by which these agonists induce bronchodilatation is thought to involve airway sensory nerves. In this study, the expression and function of dopamine D 2 receptor were examined in sensory ganglia supplying the airways; Neuronal dopamine D2 receptor mRNA expression was demonstrated by single-cell RT-PCR following laser-assisted microdissection. The projection of the neurons to the airways was confirmed by retrograde neuronal labeling. In functional studies, dopamine D2 receptor agonists (AR-C65116AB and ropinirole) inhibited intraneuronal calcium mobilization in rat capsaicin-sensitive primary sensory neurons and capsaicin-induced plasma extravasation in the rat trachea. Our results provide support to the hypothesis that dopamine D2 receptor activation inhibits neurogenic inflammation and proinflammatory reflex responses.

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Dopamine type 2 receptor expression and function in rodent sensory neurons projecting to the airways

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