Duck hepatitis B virus virion secretion requires a double-stranded DNA genome

David Perlman, Jianming Hu

Research output: Contribution to journalArticlepeer-review

44 Scopus citations


Hepatitis B virus assembly begins with the packaging of an RNA pregenome into intracellular nucleocapsids, with subsequent reverse transcription within these nucleocapsids converting the RNA into a characteristic, partially double-stranded DNA, which, alone, is found in enveloped extracellular virions as the viral genome. Using a synchronized replication system for the duck hepatitis B virus, together with a stringent two-step assay for virion secretion, we demonstrate that this selective genome secretion results from an intrinsic secretion competence gained only by the nucleocapsids at the late stage of reverse transcription.

Original languageEnglish (US)
Pages (from-to)2287-2294
Number of pages8
JournalJournal of virology
Issue number3
StatePublished - Feb 2003

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology


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