TY - JOUR
T1 - Duplicated zinc finger protein genes on the proximal short arm of the human x chromosome
T2 - Isolation, characterization and x-inactivation studies
AU - Grelg, Gillian M.
AU - Sharp, Cecll B.
AU - Carrel, Laura
AU - Willard, Huntington F.
N1 - Funding Information:
The authors are grateful to M.Mahtani for early contributions to this study and to C.Brown and R.Lafreniere for materials and helpful discussions. This work was supported by research grant GM45441 from die National Institutes of Health.
PY - 1993/10
Y1 - 1993/10
N2 - Two highly similar zinc finger genes, ZXDA (Zinc finger, X-linked, Duplicated) and ZXDB, have been isolated and characterized. Both map to the proximal short arm region of the human X chromosome, near locus DXS422 in Xpll.21. Both genes are expressed in several human tissues, revealing a ̃6.5 kb mRNA by Northern blot hybridization, and both are subject to X-inactivation. A comparison of 1.2 kb of cDNA sequence from a single exon in the open reading frames of the two genes reveals 98.7% identity in nucleotide sequence. The predicted proteins include at least ten tandem C2-H2 zinc finger motifs. When cDNA probes from different parts of the genes are hybridized to a blot of different animal DNAs, two bands are seen in all placental mammals, suggesting that the duplication predates the radiation of placental mammals and is highly conserved. Furthermore, under conditions of lowered stringency, additional bands are seen in several species, suggesting that the two genes reported here may be members of a larger zinc finger gene family.
AB - Two highly similar zinc finger genes, ZXDA (Zinc finger, X-linked, Duplicated) and ZXDB, have been isolated and characterized. Both map to the proximal short arm region of the human X chromosome, near locus DXS422 in Xpll.21. Both genes are expressed in several human tissues, revealing a ̃6.5 kb mRNA by Northern blot hybridization, and both are subject to X-inactivation. A comparison of 1.2 kb of cDNA sequence from a single exon in the open reading frames of the two genes reveals 98.7% identity in nucleotide sequence. The predicted proteins include at least ten tandem C2-H2 zinc finger motifs. When cDNA probes from different parts of the genes are hybridized to a blot of different animal DNAs, two bands are seen in all placental mammals, suggesting that the duplication predates the radiation of placental mammals and is highly conserved. Furthermore, under conditions of lowered stringency, additional bands are seen in several species, suggesting that the two genes reported here may be members of a larger zinc finger gene family.
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U2 - 10.1093/hmg/2.10.1611
DO - 10.1093/hmg/2.10.1611
M3 - Article
C2 - 8268913
AN - SCOPUS:0027425631
SN - 0964-6906
VL - 2
SP - 1611
EP - 1618
JO - Human molecular genetics
JF - Human molecular genetics
IS - 10
ER -