TY - JOUR
T1 - Dynamic microcompartmentation in synthetic cells
AU - Long, M. Scott
AU - Jones, Clinton D.
AU - Helfrich, Marcus R.
AU - Mangeney-Slavin, Lauren K.
AU - Keating, Christine D.
PY - 2005/4/26
Y1 - 2005/4/26
N2 - An experimental model for cytoplasmic organization is presented. We demonstrate dynamic control over protein distribution within synthetic cells comprising a lipid bilayer membrane surrounding an aqueous polymer solution. This polymer solution generally exists as two immiscible aqueous phases. Protein partitioning between these phases leads to microcompartmentation, or heterogeneous protein distribution within the "cell" interior. This model cytoplasm can be reversibly converted to a single phase by slight changes in temperature or osmolarity, such that local protein concentrations can be manipulated within the vesicle interior.
AB - An experimental model for cytoplasmic organization is presented. We demonstrate dynamic control over protein distribution within synthetic cells comprising a lipid bilayer membrane surrounding an aqueous polymer solution. This polymer solution generally exists as two immiscible aqueous phases. Protein partitioning between these phases leads to microcompartmentation, or heterogeneous protein distribution within the "cell" interior. This model cytoplasm can be reversibly converted to a single phase by slight changes in temperature or osmolarity, such that local protein concentrations can be manipulated within the vesicle interior.
UR - http://www.scopus.com/inward/record.url?scp=17844409041&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17844409041&partnerID=8YFLogxK
U2 - 10.1073/pnas.0409333102
DO - 10.1073/pnas.0409333102
M3 - Article
C2 - 15788532
AN - SCOPUS:17844409041
SN - 0027-8424
VL - 102
SP - 5920
EP - 5925
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 17
ER -