TY - JOUR
T1 - Dynamic protein interactions in the bacteriophage T4 replisome
AU - Trakselis, Michael A.
AU - Mayer, M. Uljana
AU - Ishmael, Faoud T.
AU - Roccasecca, Rosa Maria
AU - Benkovic, Stephen J.
N1 - Funding Information:
We thank Stephen Alley for providing guidance on these experiments as well as Frank Salinas, Ann Valentine, Ernesto Abel-Santos and Dan Jones for helpful discussions. Work in our laboratory is supported by National Institutes of Health Grants GM13306 (S.J.B.) and DK19691 (F.T.I.) as well as a grant from The Jane Coffin Childs Memorial Fund for Medical Research (M.U.M.).
PY - 2001/9/1
Y1 - 2001/9/1
N2 - The bacteriophage T4 DNA replisome is a complex dynamic system employing a variety of proteins to orchestrate the synthesis of DNA on both the leading and lagging strands. Assembly of the protein complexes responsible for DNA synthesis and priming requires the coordination of transient biomolecular interactions. This interplay of proteins has been dissected through the use of small molecules including fluorescent probes and crosslinkers, enabling the development of a complex dynamic structural and kinetic model for DNA polymerase holoenzyme assembly and primosome formation.
AB - The bacteriophage T4 DNA replisome is a complex dynamic system employing a variety of proteins to orchestrate the synthesis of DNA on both the leading and lagging strands. Assembly of the protein complexes responsible for DNA synthesis and priming requires the coordination of transient biomolecular interactions. This interplay of proteins has been dissected through the use of small molecules including fluorescent probes and crosslinkers, enabling the development of a complex dynamic structural and kinetic model for DNA polymerase holoenzyme assembly and primosome formation.
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U2 - 10.1016/S0968-0004(01)01929-6
DO - 10.1016/S0968-0004(01)01929-6
M3 - Review article
C2 - 11551794
AN - SCOPUS:0035449555
SN - 0968-0004
VL - 26
SP - 566
EP - 572
JO - Trends in Biochemical Sciences
JF - Trends in Biochemical Sciences
IS - 9
ER -