TY - JOUR
T1 - Dynamics of GATA1 binding and expression response in a GATA1-induced erythroid differentiation system
AU - Jain, Deepti
AU - Mishra, Tejaswini
AU - Giardine, Belinda M.
AU - Keller, Cheryl A.
AU - Morrissey, Christapher S.
AU - Magargee, Susan
AU - Dorman, Christine M.
AU - Long, Maria
AU - Weiss, Mitchell J.
AU - Hardison, Ross C.
N1 - Funding Information:
We thank Daniela I. Drautz for sequencing libraries on Illumina Genome sequencer (GAIIx) and Anshul Kundaje for generating the cross-correlation scores for the mouse ENCODE ChIP-seq samples. This work was supported by the National Institutes of Health grants R01DK065806 (RCH, MJW, GAB), U01HL099656 and P30DK090969 (MJW), RC2HG005573, R56DK065806, and U54HG006998 (RCH). This work was supported in part through instrumentation funded by the National Science Foundation through grant OCI-0821527 (the Penn State CyberSTAR and BioSTAR computers).
Publisher Copyright:
© 2015 The Authors.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - During the maturation phase of mammalian erythroid differentiation, highly proliferative cells committed to the erythroid lineage undergo dramatic changes in morphology and function to produce circulating, enucleated erythrocytes. These changes are caused by equally dramatic alterations in gene expression, which in turn are driven by changes in the abundance and binding patterns of transcription factors such as GATA1. We have studied the dynamics of GATA1 binding by ChIP-seq and the global expression responses by RNA-seq in a GATA1-dependent mouse cell line model for erythroid maturation, in both cases examining seven progressive stages during differentiation. Analyses of these data should provide insights both into mechanisms of regulation (early versus late targets) and the consequences in cell physiology (e.g., distinctive categories of genes regulated at progressive stages of differentiation). The data are deposited in the Gene Expression Omnibus, series GSE36029, GSE40522, GSE49847, and GSE51338.
AB - During the maturation phase of mammalian erythroid differentiation, highly proliferative cells committed to the erythroid lineage undergo dramatic changes in morphology and function to produce circulating, enucleated erythrocytes. These changes are caused by equally dramatic alterations in gene expression, which in turn are driven by changes in the abundance and binding patterns of transcription factors such as GATA1. We have studied the dynamics of GATA1 binding by ChIP-seq and the global expression responses by RNA-seq in a GATA1-dependent mouse cell line model for erythroid maturation, in both cases examining seven progressive stages during differentiation. Analyses of these data should provide insights both into mechanisms of regulation (early versus late targets) and the consequences in cell physiology (e.g., distinctive categories of genes regulated at progressive stages of differentiation). The data are deposited in the Gene Expression Omnibus, series GSE36029, GSE40522, GSE49847, and GSE51338.
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U2 - 10.1016/j.gdata.2015.01.008
DO - 10.1016/j.gdata.2015.01.008
M3 - Article
AN - SCOPUS:84929394456
SN - 2213-5960
VL - 4
SP - 1
EP - 7
JO - Genomics Data
JF - Genomics Data
ER -