TY - JOUR
T1 - Early avian follicular development is characterized by changes in transcripts involved in steroidogenesis, paracrine signaling and transcription
AU - Diaz, Francisco J.
AU - Anthony, Kate
AU - Halfhill, Ashley N.
PY - 2011/3
Y1 - 2011/3
N2 - The mechanisms associated with follicular activation and early growth are not well understood in avian species. Level of mRNA transcripts involved in steroidogenesis (STAR, HSD3B, CYP11A1, CYP19), paracrine signaling (AMH, BMP2, BMP4, BMP6, KITL, WNT4, and PCSK6) and transcription (SMAD1, SMAD2, SMAD3, SMAD5, SMAD9, FOXL3, FOXL2, NR5A1 (SF1), and WT1) were determined in small avian follicles 0.5, 1, and 2mm in diameter after oocyte removal. STAR, HSD3B, CYP11A1, CYP19, PCSK6, FOXO3, and KITL mRNA increased 2- to 45-fold, while FOXL2, WT1, and WNT4 decreased 30-90% and NR5A1 did not change as follicles developed from 0.5 to 2mm. Phosphorylated SMAD2, SMAD3, SMAD1/5/9 and FOXO3 proteins were found in both granulosa cells and oocytes of small (<0.5mm) and larger (>1mm) follicles. In contrast, non-phosphorylated FOXO3 protein was found in oocyte and granulosa cells of small follicles, but only in the oocyte of larger follicles. Culture of small avian follicles on the chorioallantoic membrane of chick embryos (in ovo) for 7 days caused changes in transcript levels that were similar to changes observed in vivo. The collective findings suggest that the growth of avian follicles from 0.5 to 2mm is marked by an increase in steroidogenic capacity, and changes in paracrine signaling that may be important during early avian follicular development. Thus, a number of candidate marker genes were identified, and a method of follicle culture was developed to study early follicular development in a model avian species.
AB - The mechanisms associated with follicular activation and early growth are not well understood in avian species. Level of mRNA transcripts involved in steroidogenesis (STAR, HSD3B, CYP11A1, CYP19), paracrine signaling (AMH, BMP2, BMP4, BMP6, KITL, WNT4, and PCSK6) and transcription (SMAD1, SMAD2, SMAD3, SMAD5, SMAD9, FOXL3, FOXL2, NR5A1 (SF1), and WT1) were determined in small avian follicles 0.5, 1, and 2mm in diameter after oocyte removal. STAR, HSD3B, CYP11A1, CYP19, PCSK6, FOXO3, and KITL mRNA increased 2- to 45-fold, while FOXL2, WT1, and WNT4 decreased 30-90% and NR5A1 did not change as follicles developed from 0.5 to 2mm. Phosphorylated SMAD2, SMAD3, SMAD1/5/9 and FOXO3 proteins were found in both granulosa cells and oocytes of small (<0.5mm) and larger (>1mm) follicles. In contrast, non-phosphorylated FOXO3 protein was found in oocyte and granulosa cells of small follicles, but only in the oocyte of larger follicles. Culture of small avian follicles on the chorioallantoic membrane of chick embryos (in ovo) for 7 days caused changes in transcript levels that were similar to changes observed in vivo. The collective findings suggest that the growth of avian follicles from 0.5 to 2mm is marked by an increase in steroidogenic capacity, and changes in paracrine signaling that may be important during early avian follicular development. Thus, a number of candidate marker genes were identified, and a method of follicle culture was developed to study early follicular development in a model avian species.
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U2 - 10.1002/mrd.21288
DO - 10.1002/mrd.21288
M3 - Article
C2 - 21308853
AN - SCOPUS:79952770457
SN - 1040-452X
VL - 78
SP - 212
EP - 223
JO - Molecular reproduction and development
JF - Molecular reproduction and development
IS - 3
ER -