TY - JOUR
T1 - Ectopic expression of the floral homeotic gene AGAMOUS in transgenic Arabidopsis plants alters floral organ identity
AU - Mizukami, Yukiko
AU - Ma, Hong
N1 - Funding Information:
We would like to thank H. Huang for developing the modified procedure of Agrobacterium-mediated transformation of Arabidopsis root explants, M. F. Yanofsky, S. A. Kempin, and M. A. Mandel for communicating unpublished data, H. J. Klee for the pMON530 vector and the ASE Agrobacterium strain, G. Rudomen for help with the scanning electron microscopy, and C. A. Flanagan, M. Z. Gilman, E. Grotewold, H. Huang, R. A. Martienssen, E. J. Richards, and C. A. Weiss for helpful discussion and comments on this manuscript. This work was supported by funds from the Robertson Fund and by a grant from the National Science Foundation (DCB-9105260) to H. M. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC Section 1734 solely to indicate this fact.
PY - 1992/10/2
Y1 - 1992/10/2
N2 - The Arabidopsis floral homeotic gene AGAMOUS (AG) is required for development of the reproductive organs (stamens and carpels). In ag mutants, the loss of AG function leads to the conversion of these organs to the perianth organs (petals and sepals). In contrast, mutations in another floral homeotic gene, APETALA2 (AP2), result in the replacement of the perianth organs by the reproductive organs. On the basis of these observations, it has been proposed that AG and AP2 act in an antagonistic fashion. To test this hypothesis, we have studied the effects of ectopically expressed AG in transgenic Arabidopsis plants. The flowers of the transgenic plants exhibit a range of phenotypes mirroring those of ap2 mutants. These experiments provide direct evidence of the proposed antagonism between AG and AP2 functions, and the results strongly suggest that AG does indeed inhibit AP2 function.
AB - The Arabidopsis floral homeotic gene AGAMOUS (AG) is required for development of the reproductive organs (stamens and carpels). In ag mutants, the loss of AG function leads to the conversion of these organs to the perianth organs (petals and sepals). In contrast, mutations in another floral homeotic gene, APETALA2 (AP2), result in the replacement of the perianth organs by the reproductive organs. On the basis of these observations, it has been proposed that AG and AP2 act in an antagonistic fashion. To test this hypothesis, we have studied the effects of ectopically expressed AG in transgenic Arabidopsis plants. The flowers of the transgenic plants exhibit a range of phenotypes mirroring those of ap2 mutants. These experiments provide direct evidence of the proposed antagonism between AG and AP2 functions, and the results strongly suggest that AG does indeed inhibit AP2 function.
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U2 - 10.1016/0092-8674(92)90271-D
DO - 10.1016/0092-8674(92)90271-D
M3 - Article
C2 - 1356630
AN - SCOPUS:0026649411
SN - 0092-8674
VL - 71
SP - 119
EP - 131
JO - Cell
JF - Cell
IS - 1
ER -