Effect of 2-chloro-4-methylthiobutanoic acid in a rapid bioassay for gastric carcinogens

Chie Furihata; Masashi Oka; Shantu Amin; Jacek Krzeminski; John H. Weisburger; Kiyoshi Kobayashi; Masae Tatematsu

doi:10.1016/S0304-3835(96)04404-7

Effect of 2-chloro-4-methylthiobutanoic acid in a rapid bioassay for gastric carcinogens

Chie Furihata, Masashi Oka, Shantu Amin, Jacek Krzeminski, John H. Weisburger, Kiyoshi Kobayashi, Masae Tatematsu

Research output: Contribution to journal › Article › peer-review

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Abstract

2-Chloro-4-methylthiobutanoic acid (CMBA, a mutagen from Japanese salted fish) at 15-500 mg/kg body weight induced several-fold increase in replicative DNA synthesis (RDS) (P < 0.05) after 80 min and 17 h, equivocal unscheduled DNA synthesis (UDS) after 80 min and necrosis 80 min after its administration in the stomach pyloric mucosa of F344 and ACI male rats. A positive control, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG, 50 mg/kg body weight), induced RDS, UDS and erosion. However, the negative control L-methionine (500 mg/kg body weight) did not display any effect. The results suggest possible tumor-initiating and -promoting activity of CMBA but at a lower potency than that of MNNG.

Original language	English (US)
Pages (from-to)	129-135
Number of pages	7
Journal	Cancer Letters
Volume	108
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3835(96)04404-7
State	Published - Nov 12 1996

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Oncology
Cancer Research

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10.1016/S0304-3835(96)04404-7

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title = "Effect of 2-chloro-4-methylthiobutanoic acid in a rapid bioassay for gastric carcinogens",

abstract = "2-Chloro-4-methylthiobutanoic acid (CMBA, a mutagen from Japanese salted fish) at 15-500 mg/kg body weight induced several-fold increase in replicative DNA synthesis (RDS) (P < 0.05) after 80 min and 17 h, equivocal unscheduled DNA synthesis (UDS) after 80 min and necrosis 80 min after its administration in the stomach pyloric mucosa of F344 and ACI male rats. A positive control, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG, 50 mg/kg body weight), induced RDS, UDS and erosion. However, the negative control L-methionine (500 mg/kg body weight) did not display any effect. The results suggest possible tumor-initiating and -promoting activity of CMBA but at a lower potency than that of MNNG.",

author = "Chie Furihata and Masashi Oka and Shantu Amin and Jacek Krzeminski and Weisburger, {John H.} and Kiyoshi Kobayashi and Masae Tatematsu",

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doi = "10.1016/S0304-3835(96)04404-7",

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T1 - Effect of 2-chloro-4-methylthiobutanoic acid in a rapid bioassay for gastric carcinogens

AU - Furihata, Chie

AU - Oka, Masashi

AU - Amin, Shantu

AU - Krzeminski, Jacek

AU - Weisburger, John H.

AU - Kobayashi, Kiyoshi

AU - Tatematsu, Masae

PY - 1996/11/12

Y1 - 1996/11/12

N2 - 2-Chloro-4-methylthiobutanoic acid (CMBA, a mutagen from Japanese salted fish) at 15-500 mg/kg body weight induced several-fold increase in replicative DNA synthesis (RDS) (P < 0.05) after 80 min and 17 h, equivocal unscheduled DNA synthesis (UDS) after 80 min and necrosis 80 min after its administration in the stomach pyloric mucosa of F344 and ACI male rats. A positive control, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG, 50 mg/kg body weight), induced RDS, UDS and erosion. However, the negative control L-methionine (500 mg/kg body weight) did not display any effect. The results suggest possible tumor-initiating and -promoting activity of CMBA but at a lower potency than that of MNNG.

AB - 2-Chloro-4-methylthiobutanoic acid (CMBA, a mutagen from Japanese salted fish) at 15-500 mg/kg body weight induced several-fold increase in replicative DNA synthesis (RDS) (P < 0.05) after 80 min and 17 h, equivocal unscheduled DNA synthesis (UDS) after 80 min and necrosis 80 min after its administration in the stomach pyloric mucosa of F344 and ACI male rats. A positive control, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG, 50 mg/kg body weight), induced RDS, UDS and erosion. However, the negative control L-methionine (500 mg/kg body weight) did not display any effect. The results suggest possible tumor-initiating and -promoting activity of CMBA but at a lower potency than that of MNNG.

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JO - Cancer Letters

JF - Cancer Letters

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ER -

Effect of 2-chloro-4-methylthiobutanoic acid in a rapid bioassay for gastric carcinogens

Abstract

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