TY - GEN
T1 - Effect of enzymatic digestion on the transverse mechanical properties of annulus fibrosus lamellae
AU - Isaacs, J. L.
AU - Vresilovic, E.
AU - Marcolongo, M.
PY - 2010/6/8
Y1 - 2010/6/8
N2 - Intervertebral disc (IVD) degeneration is associated with changes in the major biomolecular components in the disc as well as changes in enzymatic activity in the IVD. Collagen, elastin, and proteoglycans in the disc are primarily responsible for mechanical and structural support as well as disc recovery after loading. In order to investigate the effects of the loss of these biomolecules, a single annulus fibrosus (AF) lamellar model has been developed. Isolated segments of human AF were digested with elastase, collagenase and chondroitinase ABC to selectively degenerate collagen, elastin, and proteoglycan respectively. Samples were then mechanically tested to determine the contribution of these components to the mechanical properties. Understanding the effects of biomolecular changes in the IVD will promote understanding of the relationship between composition and mechanical behavior.
AB - Intervertebral disc (IVD) degeneration is associated with changes in the major biomolecular components in the disc as well as changes in enzymatic activity in the IVD. Collagen, elastin, and proteoglycans in the disc are primarily responsible for mechanical and structural support as well as disc recovery after loading. In order to investigate the effects of the loss of these biomolecules, a single annulus fibrosus (AF) lamellar model has been developed. Isolated segments of human AF were digested with elastase, collagenase and chondroitinase ABC to selectively degenerate collagen, elastin, and proteoglycan respectively. Samples were then mechanically tested to determine the contribution of these components to the mechanical properties. Understanding the effects of biomolecular changes in the IVD will promote understanding of the relationship between composition and mechanical behavior.
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U2 - 10.1109/NEBC.2010.5458160
DO - 10.1109/NEBC.2010.5458160
M3 - Conference contribution
AN - SCOPUS:77953054890
SN - 9781424468799
T3 - Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010
BT - Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010
T2 - 36th Annual Northeast Bioengineering Conference, NEBEC 2010
Y2 - 26 March 2010 through 28 March 2010
ER -