TY - JOUR
T1 - Effect of interfacial properties on the reactivity of a lipophilic ingredient in multilayered emulsions
AU - Chaprenet, Jérôme
AU - Berton-Carabin, Claire C.
AU - Elias, Ryan J.
AU - Coupland, John N.
N1 - Publisher Copyright:
© 2013 Elsevier Ltd.
PY - 2014/12/15
Y1 - 2014/12/15
N2 - The aim of this work was to investigate the location and reactivity of a lipophilic spin probe, 4-phenyl-2,2,5,5-tetramethyl-3-imidazoline-1-oxyl nitroxide (PTMIO) in multilayered, biopolymer-based emulsions stabilized with a primary anionic layer (sodium caseinate) and a secondary cationic layer (lysozyme or diethylaminoethyl (DEAE) dextran). A broad range of ζ-potential values, from ca. -55 mV to 35 mV, was achieved. Emulsions with good physical stability were achieved when the magnitude of the net charge on the droplets was sufficiently great, otherwise some physical destabilization (flocculation) could be observed, especially in the case of caseinate-lysozyme-stabilized emulsions. The analysis of electron paramagnetic resonance (EPR) spectra of PTMIO in emulsion systems showed that probe molecules partitioned between the oil droplet core (ca. 73%) and the aqueous phase (ca. 27%), independently of the interfacial composition. Surprisingly, the rate of reduction of the nitroxide group of PTMIO by ascorbate anions remained unchanged when secondary interfacial layers were added, showing that the droplet surface charge was not the prevalent factor controlling the interactions between lipophilic compounds and aqueous phase reagents. Instead we argue that the reduction of PTMIO occurs in the aqueous phase.
AB - The aim of this work was to investigate the location and reactivity of a lipophilic spin probe, 4-phenyl-2,2,5,5-tetramethyl-3-imidazoline-1-oxyl nitroxide (PTMIO) in multilayered, biopolymer-based emulsions stabilized with a primary anionic layer (sodium caseinate) and a secondary cationic layer (lysozyme or diethylaminoethyl (DEAE) dextran). A broad range of ζ-potential values, from ca. -55 mV to 35 mV, was achieved. Emulsions with good physical stability were achieved when the magnitude of the net charge on the droplets was sufficiently great, otherwise some physical destabilization (flocculation) could be observed, especially in the case of caseinate-lysozyme-stabilized emulsions. The analysis of electron paramagnetic resonance (EPR) spectra of PTMIO in emulsion systems showed that probe molecules partitioned between the oil droplet core (ca. 73%) and the aqueous phase (ca. 27%), independently of the interfacial composition. Surprisingly, the rate of reduction of the nitroxide group of PTMIO by ascorbate anions remained unchanged when secondary interfacial layers were added, showing that the droplet surface charge was not the prevalent factor controlling the interactions between lipophilic compounds and aqueous phase reagents. Instead we argue that the reduction of PTMIO occurs in the aqueous phase.
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U2 - 10.1016/j.foodhyd.2013.12.009
DO - 10.1016/j.foodhyd.2013.12.009
M3 - Article
AN - SCOPUS:84891451451
SN - 0268-005X
VL - 42
SP - 56
EP - 65
JO - Food Hydrocolloids
JF - Food Hydrocolloids
ER -