Effects of Abiotic Inducers on Sesquiterpene Synthesis in Hairy Root and Cell-Suspension Cultures of Hyoscyamus muticus

Ülkü Mehmetoǧlu; Wayne R. Curtis

doi:10.1007/BF02787842

Effects of Abiotic Inducers on Sesquiterpene Synthesis in Hairy Root and Cell-Suspension Cultures of Hyoscyamus muticus

Ülkü Mehmetoǧlu, Wayne R. Curtis

Chemical Engineering

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The effects of hydrogen peroxide (H₂O₂), salicylic acid (SA), ethanol (EtOH), and methyl jasmonate (MJ) on the lubimin and solavetivone production in hairy root and cell suspension cultures of Hyoscyamus muticus were investigated. H₂O₂ (0.194-1.94) mM did not show any positive effect of the lubimin and solavetivone production. Lubimin production of cell-suspension cultures increased 50% in the presence of 40 μM SA. For hairy root cultures, lubimin and solavetivone production increased 5 and 48%, respectively, in the presence of 4 μM SA ^•EtOH (20 mM) increased the lubimin and solavetivone concentrations 13% for hairy roots suggesting a potential rule for wounding or membrane disruption. MJ (4 μM) and 20 mM EtOH increased the lubimin and solavetivone concentrations 25 and 85%, respectively for hairy roots of H. muticus.

Original language	English (US)
Pages (from-to)	71-77
Number of pages	7
Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume	67
Issue number	1-2
DOIs	https://doi.org/10.1007/BF02787842
State	Published - Jan 1 1997

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology

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title = "Effects of Abiotic Inducers on Sesquiterpene Synthesis in Hairy Root and Cell-Suspension Cultures of Hyoscyamus muticus",

abstract = "The effects of hydrogen peroxide (H2O2), salicylic acid (SA), ethanol (EtOH), and methyl jasmonate (MJ) on the lubimin and solavetivone production in hairy root and cell suspension cultures of Hyoscyamus muticus were investigated. H2O2 (0.194-1.94) mM did not show any positive effect of the lubimin and solavetivone production. Lubimin production of cell-suspension cultures increased 50% in the presence of 40 μM SA. For hairy root cultures, lubimin and solavetivone production increased 5 and 48%, respectively, in the presence of 4 μM SA •EtOH (20 mM) increased the lubimin and solavetivone concentrations 13% for hairy roots suggesting a potential rule for wounding or membrane disruption. MJ (4 μM) and 20 mM EtOH increased the lubimin and solavetivone concentrations 25 and 85%, respectively for hairy roots of H. muticus.",

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year = "1997",

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T1 - Effects of Abiotic Inducers on Sesquiterpene Synthesis in Hairy Root and Cell-Suspension Cultures of Hyoscyamus muticus

AU - Mehmetoǧlu, Ülkü

AU - Curtis, Wayne R.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - The effects of hydrogen peroxide (H2O2), salicylic acid (SA), ethanol (EtOH), and methyl jasmonate (MJ) on the lubimin and solavetivone production in hairy root and cell suspension cultures of Hyoscyamus muticus were investigated. H2O2 (0.194-1.94) mM did not show any positive effect of the lubimin and solavetivone production. Lubimin production of cell-suspension cultures increased 50% in the presence of 40 μM SA. For hairy root cultures, lubimin and solavetivone production increased 5 and 48%, respectively, in the presence of 4 μM SA •EtOH (20 mM) increased the lubimin and solavetivone concentrations 13% for hairy roots suggesting a potential rule for wounding or membrane disruption. MJ (4 μM) and 20 mM EtOH increased the lubimin and solavetivone concentrations 25 and 85%, respectively for hairy roots of H. muticus.

AB - The effects of hydrogen peroxide (H2O2), salicylic acid (SA), ethanol (EtOH), and methyl jasmonate (MJ) on the lubimin and solavetivone production in hairy root and cell suspension cultures of Hyoscyamus muticus were investigated. H2O2 (0.194-1.94) mM did not show any positive effect of the lubimin and solavetivone production. Lubimin production of cell-suspension cultures increased 50% in the presence of 40 μM SA. For hairy root cultures, lubimin and solavetivone production increased 5 and 48%, respectively, in the presence of 4 μM SA •EtOH (20 mM) increased the lubimin and solavetivone concentrations 13% for hairy roots suggesting a potential rule for wounding or membrane disruption. MJ (4 μM) and 20 mM EtOH increased the lubimin and solavetivone concentrations 25 and 85%, respectively for hairy roots of H. muticus.

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M3 - Article

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JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

IS - 1-2

ER -

Effects of Abiotic Inducers on Sesquiterpene Synthesis in Hairy Root and Cell-Suspension Cultures of Hyoscyamus muticus

Abstract

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