TY - JOUR
T1 - Effects of Delignification on Crystalline Cellulose in Lignocellulose Biomass Characterized by Vibrational Sum Frequency Generation Spectroscopy and X-ray Diffraction
AU - Kafle, Kabindra
AU - Lee, Christopher M.
AU - Shin, Heenae
AU - Zoppe, Justin
AU - Johnson, David K.
AU - Kim, Seong H.
AU - Park, Sunkyu
N1 - Funding Information:
This work was supported by Subcontract No. XGB-3-23024-01 with the National Renewable Energy Laboratory, under Prime Contract No. DE-AC36-08-GO28308 with the US Department of Energy. The portion of the sample preparation and XRD work was supported by National Research Foundation of Korea under Award No. 2011-0025029.
Publisher Copyright:
© 2015, Springer Science+Business Media New York.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Delignification, a common practice in the pulping industry, has been proposed and explored as a means to selectively remove lignin from lignocellulosic biomass and, thus, increase enzyme accessibility for cellulose hydrolysis. However, without knowing structural changes of cellulose in biomass, it is difficult to fully understand the effects of the delignification process on cellulose hydrolysis. In this study, the amount and aggregation of crystalline cellulose in hardwood biomass delignified using oxygen and sodium chlorite as reactive agents were examined with vibrational sum frequency generation (SFG) spectroscopy and X-ray diffraction (XRD). The results indicated that the amount of crystalline cellulose and the XRD crystallite size increased with both oxygen and chlorite delignification processes. In addition, the “α-cellulose equivalent” fraction estimated by SFG spectroscopy increased greater than glucan amount with the delignification process. Changes in crystal size might be due to the aggregation of cellulose crystals, along with the increase in crystalline cellulose amount.
AB - Delignification, a common practice in the pulping industry, has been proposed and explored as a means to selectively remove lignin from lignocellulosic biomass and, thus, increase enzyme accessibility for cellulose hydrolysis. However, without knowing structural changes of cellulose in biomass, it is difficult to fully understand the effects of the delignification process on cellulose hydrolysis. In this study, the amount and aggregation of crystalline cellulose in hardwood biomass delignified using oxygen and sodium chlorite as reactive agents were examined with vibrational sum frequency generation (SFG) spectroscopy and X-ray diffraction (XRD). The results indicated that the amount of crystalline cellulose and the XRD crystallite size increased with both oxygen and chlorite delignification processes. In addition, the “α-cellulose equivalent” fraction estimated by SFG spectroscopy increased greater than glucan amount with the delignification process. Changes in crystal size might be due to the aggregation of cellulose crystals, along with the increase in crystalline cellulose amount.
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U2 - 10.1007/s12155-015-9627-9
DO - 10.1007/s12155-015-9627-9
M3 - Article
AN - SCOPUS:84947041044
SN - 1939-1234
VL - 8
SP - 1750
EP - 1758
JO - Bioenergy Research
JF - Bioenergy Research
IS - 4
ER -
