TY - JOUR
T1 - Effects of nicotine and cotinine on bovine theca intema and granulosa cells
AU - Sanders, Sara R.
AU - Cuneo, S. Peder
AU - Turzillo, Adele M.
N1 - Funding Information:
We thank Sandra Dugas for technical assistance and the University of Arizona Dairy Research Facility for providing daily care and management of the animals used in this study. We also thank Dr. Patricia B. Hoyer for helpful comments and suggestions during preparation of this manuscript. This work was supported by a grant from the Arizona Disease Control Research Commission (Contract number 5008).
PY - 2002/11
Y1 - 2002/11
N2 - The purpose of this study was to determine if nicotine or cotinine inhibits steroidogenesis in the ovarian follicle. Theca interna and granulosa cells were isolated from bovine follicles, cultured with nicotine or cotinine for 24 h, and culture media were assayed for androstenedione or estradiol. Treatment of theca interna with 6, 60, and 600 μM nicotine decreased (P ≤ 0.002) production of androstenedione to 55, 53, and 24% of control levels, respectively. Levels of androstenedione in theca interna treated with cotinine were not different from control values. In granulosa cells, nicotine inhibited production of estradiol at the highest dose tested. Treatment with 600 μM nicotine decreased (P ≤ 0.001) estradiol concentration to 12% of control values, attributable to a general cytotoxic effect. Cotinine had no effect on estradiol production by granulosa cells. These results provide novel evidence for inhibitory effects of nicotine on androgen production by theca interna.
AB - The purpose of this study was to determine if nicotine or cotinine inhibits steroidogenesis in the ovarian follicle. Theca interna and granulosa cells were isolated from bovine follicles, cultured with nicotine or cotinine for 24 h, and culture media were assayed for androstenedione or estradiol. Treatment of theca interna with 6, 60, and 600 μM nicotine decreased (P ≤ 0.002) production of androstenedione to 55, 53, and 24% of control levels, respectively. Levels of androstenedione in theca interna treated with cotinine were not different from control values. In granulosa cells, nicotine inhibited production of estradiol at the highest dose tested. Treatment with 600 μM nicotine decreased (P ≤ 0.001) estradiol concentration to 12% of control values, attributable to a general cytotoxic effect. Cotinine had no effect on estradiol production by granulosa cells. These results provide novel evidence for inhibitory effects of nicotine on androgen production by theca interna.
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U2 - 10.1016/S0890-6238(02)00049-7
DO - 10.1016/S0890-6238(02)00049-7
M3 - Article
C2 - 12401508
AN - SCOPUS:0036840283
SN - 0890-6238
VL - 16
SP - 795
EP - 800
JO - Reproductive Toxicology
JF - Reproductive Toxicology
IS - 6
ER -