TY - JOUR
T1 - Effects of temperature on growth of Metarhizium flavoviride and virulence to the variegated grasshopper, Zonocerus variegatus
AU - Thomas, Matthew B.
AU - Jenkins, Nina E.
N1 - Funding Information:
We are very grateful to members of the LUBILOSA team for assistance in the laboratory. We also thank Mark Goettel for permission to cite results from an abstract for the Society of Invertebrate Pathology 28th Annual Meeting, Ithaca, New York, 1995. This study forms part of the research programme of the NERC Centre for Population Biology and the LUBILOSA programme and was funded in part by a grant from The Leverhulme Trust (Grant No. F58Z). LUBILOSA is a research programme on the biological control of locusts and grasshoppers executed by the International Institute of Biological Control, of CAB INTERNATIONAL ; the International Institute of Tropical Agriculture Plant Health Management Division ; Centre pour Agronomie, Hydrologie, Meteorologie of the Comite! Inter-E; tat de Lutte contre la Secheresse au Sahel (AGRHYMET-CILSS) and ; Deutsche Gesellschaft fu$ r Technische Zusammenarbeit (GTZ) GmbH. LUBILOSA is funded by the governments of Canada (CIDA), U.K. (ODA), the Netherlands (DGIS) and Switzerland (SDC).
PY - 1997
Y1 - 1997
N2 - The temperature optima for germination, radial hyphal extension rate and sporulation were determined for two isolates of Metarhizium flavoviride from acridoid hosts. 191-609 exhibited lower temperature optima for hyphal extension (25.5 °C) and sporulation (24°) than IMI 330189 (27°and 25°, respectively), although temperature optima for germination were similar (ca 30°). The temperature optimum for sporulation was lower than that for hyphal extension. Further studies were carried out on 191-609 to investigate the effect of temperature and dose on the infection mortality rate of the grasshopper pest, Zonocerus variegatus. Increasing dose resulted in a decrease in mean survival time regardless of incubation temperature. Dose and temperature were shown to interact, with the effects of dose increasing at temperatures either side of the optimum. Furthermore, for each dose, the optimum temperature for infection was estimated to be ca 5°higher than the optimum for growth, and no significant relationship was identified between hyphal extension rate in vitro and host survival time. The implications of these results are discussed with regard to the selection of isolates for use in biological control.
AB - The temperature optima for germination, radial hyphal extension rate and sporulation were determined for two isolates of Metarhizium flavoviride from acridoid hosts. 191-609 exhibited lower temperature optima for hyphal extension (25.5 °C) and sporulation (24°) than IMI 330189 (27°and 25°, respectively), although temperature optima for germination were similar (ca 30°). The temperature optimum for sporulation was lower than that for hyphal extension. Further studies were carried out on 191-609 to investigate the effect of temperature and dose on the infection mortality rate of the grasshopper pest, Zonocerus variegatus. Increasing dose resulted in a decrease in mean survival time regardless of incubation temperature. Dose and temperature were shown to interact, with the effects of dose increasing at temperatures either side of the optimum. Furthermore, for each dose, the optimum temperature for infection was estimated to be ca 5°higher than the optimum for growth, and no significant relationship was identified between hyphal extension rate in vitro and host survival time. The implications of these results are discussed with regard to the selection of isolates for use in biological control.
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U2 - 10.1017/S0953756297004401
DO - 10.1017/S0953756297004401
M3 - Article
AN - SCOPUS:0031284656
SN - 0953-7562
VL - 101
SP - 1469
EP - 1474
JO - Mycological Research
JF - Mycological Research
IS - 12
ER -