Effects of the lipid peroxidation products 4-hydroxy-2-nonenal and malondialdehyde on the proliferation and morphogenetic development of in vitro plant cells

Linda K. Adams; Erica E. Benson; Harry J. Staines; David H. Bremner; Stephen Millam; Nigel Deighton

doi:10.1016/S0176-1617(99)80120-5

Effects of the lipid peroxidation products 4-hydroxy-2-nonenal and malondialdehyde on the proliferation and morphogenetic development of in vitro plant cells

Linda K. Adams, Erica E. Benson, Harry J. Staines, David H. Bremner, Stephen Millam, Nigel Deighton

Huck Institutes of the Life Sciences

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The purpose of this study was to evaluate the toxicological effects of 4-hydroxy-2-nonenal (HNE) and malondialdehyde (MDA) on in vitro plant cells using the model morphogenetic system of Daucus carota L. for the production of somatic embryos. It was found that both HNE and MDA inhibited proliferative growth and embryogenesis in D. carota cultures. However, embryogenic capacity was restored when callus cultures were transferred to embryo induction medium without HNE or MDA and the recovery of morphogenetic competence was more pronounced in cultures which had been exposed to MDA as compared to those which had been treated with HNE. Also, differential responses between embryogenic and nonembryogenic cultures were observed in relation to the delivery of aldehydes in different culture media. This paper reports, for the first time, the toxicological effects of HNE and MDA on in vitro plant cells. The significance of these findings in relation to the control of in vitro development in plant tissue cultures which are used in biotechnology programmes is discussed.

Original language	English (US)
Pages (from-to)	376-386
Number of pages	11
Journal	Journal of Plant Physiology
Volume	155
Issue number	3
DOIs	https://doi.org/10.1016/S0176-1617(99)80120-5
State	Published - Sep 1999

All Science Journal Classification (ASJC) codes

Physiology
Agronomy and Crop Science
Plant Science

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title = "Effects of the lipid peroxidation products 4-hydroxy-2-nonenal and malondialdehyde on the proliferation and morphogenetic development of in vitro plant cells",

abstract = "The purpose of this study was to evaluate the toxicological effects of 4-hydroxy-2-nonenal (HNE) and malondialdehyde (MDA) on in vitro plant cells using the model morphogenetic system of Daucus carota L. for the production of somatic embryos. It was found that both HNE and MDA inhibited proliferative growth and embryogenesis in D. carota cultures. However, embryogenic capacity was restored when callus cultures were transferred to embryo induction medium without HNE or MDA and the recovery of morphogenetic competence was more pronounced in cultures which had been exposed to MDA as compared to those which had been treated with HNE. Also, differential responses between embryogenic and nonembryogenic cultures were observed in relation to the delivery of aldehydes in different culture media. This paper reports, for the first time, the toxicological effects of HNE and MDA on in vitro plant cells. The significance of these findings in relation to the control of in vitro development in plant tissue cultures which are used in biotechnology programmes is discussed.",

author = "Adams, {Linda K.} and Benson, {Erica E.} and Staines, {Harry J.} and Bremner, {David H.} and Stephen Millam and Nigel Deighton",

note = "Funding Information: LKA, EEB, DHB, and HJS acknowledge financial support of the project by a University of Abertay Dundee Post Graduate Studentship. SM and ND acknowledge funding from the Scottish Office Agriculture, Environment and Fisheries Department (SOAEFD). The authors are grateful to the late Professor Esterbauer (University of Graz, Austria) and Dr. R. Gree (CNRS, Rennes, France) who generously provided gifts of HNE. The authors also gratefully acknowledge the technical assistance of Isobel Pimbley and Mike Black (UAD).",

year = "1999",

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language = "English (US)",

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AU - Adams, Linda K.

AU - Benson, Erica E.

AU - Staines, Harry J.

AU - Bremner, David H.

AU - Millam, Stephen

AU - Deighton, Nigel

N1 - Funding Information: LKA, EEB, DHB, and HJS acknowledge financial support of the project by a University of Abertay Dundee Post Graduate Studentship. SM and ND acknowledge funding from the Scottish Office Agriculture, Environment and Fisheries Department (SOAEFD). The authors are grateful to the late Professor Esterbauer (University of Graz, Austria) and Dr. R. Gree (CNRS, Rennes, France) who generously provided gifts of HNE. The authors also gratefully acknowledge the technical assistance of Isobel Pimbley and Mike Black (UAD).

PY - 1999/9

Y1 - 1999/9

N2 - The purpose of this study was to evaluate the toxicological effects of 4-hydroxy-2-nonenal (HNE) and malondialdehyde (MDA) on in vitro plant cells using the model morphogenetic system of Daucus carota L. for the production of somatic embryos. It was found that both HNE and MDA inhibited proliferative growth and embryogenesis in D. carota cultures. However, embryogenic capacity was restored when callus cultures were transferred to embryo induction medium without HNE or MDA and the recovery of morphogenetic competence was more pronounced in cultures which had been exposed to MDA as compared to those which had been treated with HNE. Also, differential responses between embryogenic and nonembryogenic cultures were observed in relation to the delivery of aldehydes in different culture media. This paper reports, for the first time, the toxicological effects of HNE and MDA on in vitro plant cells. The significance of these findings in relation to the control of in vitro development in plant tissue cultures which are used in biotechnology programmes is discussed.

AB - The purpose of this study was to evaluate the toxicological effects of 4-hydroxy-2-nonenal (HNE) and malondialdehyde (MDA) on in vitro plant cells using the model morphogenetic system of Daucus carota L. for the production of somatic embryos. It was found that both HNE and MDA inhibited proliferative growth and embryogenesis in D. carota cultures. However, embryogenic capacity was restored when callus cultures were transferred to embryo induction medium without HNE or MDA and the recovery of morphogenetic competence was more pronounced in cultures which had been exposed to MDA as compared to those which had been treated with HNE. Also, differential responses between embryogenic and nonembryogenic cultures were observed in relation to the delivery of aldehydes in different culture media. This paper reports, for the first time, the toxicological effects of HNE and MDA on in vitro plant cells. The significance of these findings in relation to the control of in vitro development in plant tissue cultures which are used in biotechnology programmes is discussed.

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Effects of the lipid peroxidation products 4-hydroxy-2-nonenal and malondialdehyde on the proliferation and morphogenetic development of in vitro plant cells

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