TY - JOUR
T1 - Effects of turn residues on β-hairpin folding - A molecular dynamics study
AU - Wang, Hongwu
AU - Sung, Shen Shu
PY - 1999
Y1 - 1999
N2 - Folding of β-hairpin structures of synthetic peptides has been simulated using the molecular dynamics method with a solvent-referenced potential. Two similar sequences, Ac-MQIFVKS(D)PGKTITLKV-NH2 and Ac- MQIFVKS(L)PGKTITLKV-NH2, derived from the N-terminal β-hairpin of ubiquitin, were used to study the effects of turn residues in β-hairpin folding. The simulations were carried out for 80 ns at 297 K. With extended initial conformation, the (D)P-containing peptide folded into a stable 2:2 β-hairpin conformation with a type II β-turn at (D)PG. The overall β- hairpin ratio, calculated by the DSSP algorithm, was 32.6%. With randomly generated initial conformations, the peptide also formed the stable 2:2 β- hairpin conformation. The interactions among the side chains in the 2:2 β- hairpin were almost identical to those in the native protein. These interactions reduced the solvation energy upon folding and stabilized the β- hairpin conformation. Without the solvent effect, the peptide did not fold into stable β-hairpin structures. The solvent effect is crucial for the formation of the β-hairpin conformation. The effect of the temperature has also been studied. The (L)P-containing peptide did not fold into a stable β- hairpin conformation and had a much lower β-hairpin ratio (16.6%). The (L)P- containing peptide has similar favorable side-chain interactions, but the turn formed by (L)PG does not connect well with the right-handed twist of the β-strands. For comparison, the isolated N-terminal peptide of ubiquitin, Ac- MQIFVKTLTGKTITLEV-NH2, was also simulated and its β-hairpin ratio was low, indicating that the β-hairpin in the native structure is stabilized by the interaction with the protein environment. These simulation results agreed qualitatively with the available experimental findings.
AB - Folding of β-hairpin structures of synthetic peptides has been simulated using the molecular dynamics method with a solvent-referenced potential. Two similar sequences, Ac-MQIFVKS(D)PGKTITLKV-NH2 and Ac- MQIFVKS(L)PGKTITLKV-NH2, derived from the N-terminal β-hairpin of ubiquitin, were used to study the effects of turn residues in β-hairpin folding. The simulations were carried out for 80 ns at 297 K. With extended initial conformation, the (D)P-containing peptide folded into a stable 2:2 β-hairpin conformation with a type II β-turn at (D)PG. The overall β- hairpin ratio, calculated by the DSSP algorithm, was 32.6%. With randomly generated initial conformations, the peptide also formed the stable 2:2 β- hairpin conformation. The interactions among the side chains in the 2:2 β- hairpin were almost identical to those in the native protein. These interactions reduced the solvation energy upon folding and stabilized the β- hairpin conformation. Without the solvent effect, the peptide did not fold into stable β-hairpin structures. The solvent effect is crucial for the formation of the β-hairpin conformation. The effect of the temperature has also been studied. The (L)P-containing peptide did not fold into a stable β- hairpin conformation and had a much lower β-hairpin ratio (16.6%). The (L)P- containing peptide has similar favorable side-chain interactions, but the turn formed by (L)PG does not connect well with the right-handed twist of the β-strands. For comparison, the isolated N-terminal peptide of ubiquitin, Ac- MQIFVKTLTGKTITLEV-NH2, was also simulated and its β-hairpin ratio was low, indicating that the β-hairpin in the native structure is stabilized by the interaction with the protein environment. These simulation results agreed qualitatively with the available experimental findings.
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U2 - 10.1002/(SICI)1097-0282(199912)50:7<763::AID-BIP9>3.0.CO;2-W
DO - 10.1002/(SICI)1097-0282(199912)50:7<763::AID-BIP9>3.0.CO;2-W
M3 - Article
C2 - 10547531
AN - SCOPUS:0032746985
SN - 0006-3525
VL - 50
SP - 763
EP - 776
JO - Biopolymers
JF - Biopolymers
IS - 7
ER -