TY - JOUR
T1 - Effects of wastewater microalgae harvesting methods on polyhydroxybutyrate production
AU - Rahman, Asif
AU - Anthony, Renil J.
AU - Sathish, Ashik
AU - Sims, Ronald C.
AU - Miller, Charles D.
N1 - Funding Information:
The authors would like to thank the Sustainable Waste to Bioproducts Engineering Center (SWBEC) , Synthetic Biomanufacturing Institute (SBI) , the Utah Science Technology and Research (USTAR) , and the City of Logan , UT for funding and supporting this project. The authors would also like to thank Ryan J. Putman for his assistance.
PY - 2014/3
Y1 - 2014/3
N2 - Microalgae have gained considerable attention recently as a sustainable means to produce biofuels and bioproducts. It has previously been demonstrated that single strain microalgae can be harvested and processed through a wet lipid extraction procedure (WLEP). After WLEP processing, acetone, butanol, ethanol, and biodiesel can be produced, and growth of recombinant Escherichia coli can be achieved from the microalgae. This study demonstrates the application of different wastewater microalgae harvesting techniques and processing through WLEP on the production of polyhydroxybutyrate (PHB) by E. coli. The harvesting techniques include: cationic potato starch (CPS), cationic corn starch (CCS), aluminum sulfate, and centrifugation. The microalgae-based media were used to grow E. coli to ~1013CFU/mL and produce approximately 7.8% of dry cell weight as PHB. This study demonstrates the feasibility of harvesting wastewater algae to produce PHB and the potential for bioproduct generation.
AB - Microalgae have gained considerable attention recently as a sustainable means to produce biofuels and bioproducts. It has previously been demonstrated that single strain microalgae can be harvested and processed through a wet lipid extraction procedure (WLEP). After WLEP processing, acetone, butanol, ethanol, and biodiesel can be produced, and growth of recombinant Escherichia coli can be achieved from the microalgae. This study demonstrates the application of different wastewater microalgae harvesting techniques and processing through WLEP on the production of polyhydroxybutyrate (PHB) by E. coli. The harvesting techniques include: cationic potato starch (CPS), cationic corn starch (CCS), aluminum sulfate, and centrifugation. The microalgae-based media were used to grow E. coli to ~1013CFU/mL and produce approximately 7.8% of dry cell weight as PHB. This study demonstrates the feasibility of harvesting wastewater algae to produce PHB and the potential for bioproduct generation.
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U2 - 10.1016/j.biortech.2014.01.034
DO - 10.1016/j.biortech.2014.01.034
M3 - Article
C2 - 24491426
AN - SCOPUS:84899063413
SN - 0960-8524
VL - 156
SP - 364
EP - 367
JO - Bioresource technology
JF - Bioresource technology
ER -
