Electrical molecular focusing for laser induced fluorescence based single DNA detection

Tza Huei Wang; Pak Kin Wong; Chih Ming Ho

doi:10.1109/MEMSYS.2002.984044

Electrical molecular focusing for laser induced fluorescence based single DNA detection

Tza Huei Wang, Pak Kin Wong, Chih Ming Ho

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

This paper details an electrokinetic focusing technique that significantly enhances the detection efficiency for laser induced fluorescence (LIF) based molecular sensing by concentrating fluorescence labeled molecules in a tiny probe region. 3-D electrodes were fabricated to generate wider and denser electric fields toward the probe region in the microchannel. The generated electric field is able to focus flowing DNA molecules to widths as narrow as 3 μm in a 120 μm wide channel. A single molecule detection technique is developed and the enhancement of detection efficiency by using the focusing method is demonstrated.

Original language	English (US)
Pages (from-to)	15-18
Number of pages	4
Journal	Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS)
DOIs	https://doi.org/10.1109/MEMSYS.2002.984044
State	Published - 2002

All Science Journal Classification (ASJC) codes

Mechanical Engineering
Electrical and Electronic Engineering
Control and Systems Engineering

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10.1109/MEMSYS.2002.984044

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title = "Electrical molecular focusing for laser induced fluorescence based single DNA detection",

abstract = "This paper details an electrokinetic focusing technique that significantly enhances the detection efficiency for laser induced fluorescence (LIF) based molecular sensing by concentrating fluorescence labeled molecules in a tiny probe region. 3-D electrodes were fabricated to generate wider and denser electric fields toward the probe region in the microchannel. The generated electric field is able to focus flowing DNA molecules to widths as narrow as 3 μm in a 120 μm wide channel. A single molecule detection technique is developed and the enhancement of detection efficiency by using the focusing method is demonstrated.",

author = "Wang, {Tza Huei} and Wong, {Pak Kin} and Ho, {Chih Ming}",

year = "2002",

doi = "10.1109/MEMSYS.2002.984044",

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journal = "Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS)",

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T1 - Electrical molecular focusing for laser induced fluorescence based single DNA detection

AU - Wang, Tza Huei

AU - Wong, Pak Kin

AU - Ho, Chih Ming

PY - 2002

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N2 - This paper details an electrokinetic focusing technique that significantly enhances the detection efficiency for laser induced fluorescence (LIF) based molecular sensing by concentrating fluorescence labeled molecules in a tiny probe region. 3-D electrodes were fabricated to generate wider and denser electric fields toward the probe region in the microchannel. The generated electric field is able to focus flowing DNA molecules to widths as narrow as 3 μm in a 120 μm wide channel. A single molecule detection technique is developed and the enhancement of detection efficiency by using the focusing method is demonstrated.

AB - This paper details an electrokinetic focusing technique that significantly enhances the detection efficiency for laser induced fluorescence (LIF) based molecular sensing by concentrating fluorescence labeled molecules in a tiny probe region. 3-D electrodes were fabricated to generate wider and denser electric fields toward the probe region in the microchannel. The generated electric field is able to focus flowing DNA molecules to widths as narrow as 3 μm in a 120 μm wide channel. A single molecule detection technique is developed and the enhancement of detection efficiency by using the focusing method is demonstrated.

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JO - Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS)

JF - Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS)

ER -

Electrical molecular focusing for laser induced fluorescence based single DNA detection

Abstract

All Science Journal Classification (ASJC) codes

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