Electrochemical monitoring of individual exocytotic events from the varicosities of differentiated PC12 cells

Susan E. Zerby; Andrew G. Ewing

doi:10.1016/0006-8993(95)01383-0

Electrochemical monitoring of individual exocytotic events from the varicosities of differentiated PC12 cells

Susan E. Zerby, Andrew G. Ewing

Chemistry

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Rat pheochromocytoma (PC12) cells have been used as a model of developing neurons to study exocytosis during differentiation. Upon treatment with nerve growth factor, PC12 cells become more neuronal-like. Using amperometric detection at carbon fiber microelectrodes, time-resolved exocytosis of electroactive catecholamines can be observed. The site of exocytosis has been compared for differentiated and undifferentiated cells. Upon differentiation, cells release catecholamines primarily from varicosities along their neurites with no release from the cell body. In addition, the mean vesicular content is not significantly altered upon differentiation although it appears that the distribution of vesicle content becomes more narrow. The number of release events observed also decreases as the cells become more neuronal in character. It is possible that the smaller range of vesicle dopamine content and the decreased number of release events observed after differentiation are a result of the relocation of the site of exocytosis.

Original language	English (US)
Pages (from-to)	1-10
Number of pages	10
Journal	Brain research
Volume	712
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(95)01383-0
State	Published - Mar 11 1996

All Science Journal Classification (ASJC) codes

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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@article{e57dda6427224dc4973d5bd92cdf910b,

title = "Electrochemical monitoring of individual exocytotic events from the varicosities of differentiated PC12 cells",

abstract = "Rat pheochromocytoma (PC12) cells have been used as a model of developing neurons to study exocytosis during differentiation. Upon treatment with nerve growth factor, PC12 cells become more neuronal-like. Using amperometric detection at carbon fiber microelectrodes, time-resolved exocytosis of electroactive catecholamines can be observed. The site of exocytosis has been compared for differentiated and undifferentiated cells. Upon differentiation, cells release catecholamines primarily from varicosities along their neurites with no release from the cell body. In addition, the mean vesicular content is not significantly altered upon differentiation although it appears that the distribution of vesicle content becomes more narrow. The number of release events observed also decreases as the cells become more neuronal in character. It is possible that the smaller range of vesicle dopamine content and the decreased number of release events observed after differentiation are a result of the relocation of the site of exocytosis.",

author = "Zerby, {Susan E.} and Ewing, {Andrew G.}",

note = "Funding Information: This work has been supported by grants from the Office of Naval Research and the National Science Foundation. We would like to acknowledge Guangyao Chen for providing assistance with computer programming and helpful discussions regarding data analysis, and Jenny Zhou for helpful discussions concerning cell culture.",

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T1 - Electrochemical monitoring of individual exocytotic events from the varicosities of differentiated PC12 cells

AU - Zerby, Susan E.

AU - Ewing, Andrew G.

N1 - Funding Information: This work has been supported by grants from the Office of Naval Research and the National Science Foundation. We would like to acknowledge Guangyao Chen for providing assistance with computer programming and helpful discussions regarding data analysis, and Jenny Zhou for helpful discussions concerning cell culture.

PY - 1996/3/11

Y1 - 1996/3/11

N2 - Rat pheochromocytoma (PC12) cells have been used as a model of developing neurons to study exocytosis during differentiation. Upon treatment with nerve growth factor, PC12 cells become more neuronal-like. Using amperometric detection at carbon fiber microelectrodes, time-resolved exocytosis of electroactive catecholamines can be observed. The site of exocytosis has been compared for differentiated and undifferentiated cells. Upon differentiation, cells release catecholamines primarily from varicosities along their neurites with no release from the cell body. In addition, the mean vesicular content is not significantly altered upon differentiation although it appears that the distribution of vesicle content becomes more narrow. The number of release events observed also decreases as the cells become more neuronal in character. It is possible that the smaller range of vesicle dopamine content and the decreased number of release events observed after differentiation are a result of the relocation of the site of exocytosis.

AB - Rat pheochromocytoma (PC12) cells have been used as a model of developing neurons to study exocytosis during differentiation. Upon treatment with nerve growth factor, PC12 cells become more neuronal-like. Using amperometric detection at carbon fiber microelectrodes, time-resolved exocytosis of electroactive catecholamines can be observed. The site of exocytosis has been compared for differentiated and undifferentiated cells. Upon differentiation, cells release catecholamines primarily from varicosities along their neurites with no release from the cell body. In addition, the mean vesicular content is not significantly altered upon differentiation although it appears that the distribution of vesicle content becomes more narrow. The number of release events observed also decreases as the cells become more neuronal in character. It is possible that the smaller range of vesicle dopamine content and the decreased number of release events observed after differentiation are a result of the relocation of the site of exocytosis.

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Electrochemical monitoring of individual exocytotic events from the varicosities of differentiated PC12 cells

Abstract

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