TY - JOUR
T1 - Electrokinetic bioprocessor for concentrating cells and molecules
AU - Wong, Pak Kin
AU - Chen, Che Yang
AU - Wang, Tza Huei
AU - Ho, Chih Ming
PY - 2005/1/3
Y1 - 2005/1/3
N2 - Bioprocessors for concentrating bioparticles, such as cells and molecules, are commonly needed in bioanalysis systems. In this microfluidic processor, a global flow field generated by ac electroosmosis transports the embedded particles to the regions near the electrode surface. The processor then utilizes electrophoretic and dielectrophoretic forces, which are effective in short range, to trap the target cells and molecules on the electrode surface. By optimizing the operating parameters, we have concentrated various biological objects in a large range of sizes, including Escherichia coli bacteria, λ phage DNA, and single-stranded DNA fragments as small as 20 bases that have a radius of gyration of only 3 nm.
AB - Bioprocessors for concentrating bioparticles, such as cells and molecules, are commonly needed in bioanalysis systems. In this microfluidic processor, a global flow field generated by ac electroosmosis transports the embedded particles to the regions near the electrode surface. The processor then utilizes electrophoretic and dielectrophoretic forces, which are effective in short range, to trap the target cells and molecules on the electrode surface. By optimizing the operating parameters, we have concentrated various biological objects in a large range of sizes, including Escherichia coli bacteria, λ phage DNA, and single-stranded DNA fragments as small as 20 bases that have a radius of gyration of only 3 nm.
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U2 - 10.1021/ac049479u
DO - 10.1021/ac049479u
M3 - Article
C2 - 15571340
AN - SCOPUS:10044278305
SN - 0003-2700
VL - 76
SP - 6908
EP - 6914
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 23
ER -