Electrophoretic and immunological detection of legumin-like seed proteins in several monocot species including several tribes of the Poaceae

SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis were used to determine the prevalence of legumin-like like proteins in seeds from plants representing nine orders of Monocotyledoneae including sixteen tribes of the Poaceae. SDS-PAGE indicated that most monocotyledonous plants examined had major storage protein fractions that could be classified as the 11 S legumin-like globulins. SDS-PAGE and Western blot analysis indicated that representatives of five tribes of the Poaceae (Graminales), Avena sativa L., Oryza sativa L., Agrostis alba L., Stipa viridula Trin. and Phalaris canariensis L. also contained legumin-like proteins as their major storage protein fraction.