TY - JOUR
T1 - Electrospray analysis of proteins
T2 - A comparison of positive‐ion and negative‐ion mass spectra at high and low pH
AU - Kelly, Michele A.
AU - Vestling, Martha M.
AU - Fenselau, Catherine C.
AU - Smith, Philip B.
PY - 1992/10
Y1 - 1992/10
N2 - Cationic and anionic electrospray mass spectra were measured for equine myoglobin, lactalbumin and hen egg white lysozyme from acidic and basic solutions. Both positive and negative ions were detected from all solutions in which the proteins were soluble. This was observed in both nebulization‐assisted and thermally assisted electrospray. As in other studies, the distribution of the charge states reflected functional groups in the proteins, and also folding or extension of the protein.
AB - Cationic and anionic electrospray mass spectra were measured for equine myoglobin, lactalbumin and hen egg white lysozyme from acidic and basic solutions. Both positive and negative ions were detected from all solutions in which the proteins were soluble. This was observed in both nebulization‐assisted and thermally assisted electrospray. As in other studies, the distribution of the charge states reflected functional groups in the proteins, and also folding or extension of the protein.
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U2 - 10.1002/oms.1210271028
DO - 10.1002/oms.1210271028
M3 - Article
AN - SCOPUS:0000547607
SN - 0030-493X
VL - 27
SP - 1143
EP - 1147
JO - Organic Mass Spectrometry
JF - Organic Mass Spectrometry
IS - 10
ER -