Encapsulation of a compartmentalized cytoplasm mimic within a lipid membrane by microfluidics

Marta Sobrinos-Sanguino; Silvia Zorrilla; Christine D. Keating; Begoña Monterroso; Germán Rivas

doi:10.1039/c7cc01289f

Encapsulation of a compartmentalized cytoplasm mimic within a lipid membrane by microfluidics

Marta Sobrinos-Sanguino, Silvia Zorrilla, Christine D. Keating, Begoña Monterroso, Germán Rivas

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

There is growing interest in analyzing the effect of microenvironments, which may be mimicked through liquid-liquid phase separation (LLPS), on the reactivity of biological macromolecules. We report the encapsulation by microfluidics of the division protein FtsZ and a LLPS system inside microdroplets and their conversion into permeable vesicles (allowing ligand uptake), with higher yield, homogeneity and biomolecular compatibility than those previously described.

Original language	English (US)
Pages (from-to)	4775-4778
Number of pages	4
Journal	Chemical Communications
Volume	53
Issue number	35
DOIs	https://doi.org/10.1039/c7cc01289f
State	Published - 2017

All Science Journal Classification (ASJC) codes

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c7cc01289f

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abstract = "There is growing interest in analyzing the effect of microenvironments, which may be mimicked through liquid-liquid phase separation (LLPS), on the reactivity of biological macromolecules. We report the encapsulation by microfluidics of the division protein FtsZ and a LLPS system inside microdroplets and their conversion into permeable vesicles (allowing ligand uptake), with higher yield, homogeneity and biomolecular compatibility than those previously described.",

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AU - Zorrilla, Silvia

AU - Keating, Christine D.

AU - Monterroso, Begoña

AU - Rivas, Germán

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AB - There is growing interest in analyzing the effect of microenvironments, which may be mimicked through liquid-liquid phase separation (LLPS), on the reactivity of biological macromolecules. We report the encapsulation by microfluidics of the division protein FtsZ and a LLPS system inside microdroplets and their conversion into permeable vesicles (allowing ligand uptake), with higher yield, homogeneity and biomolecular compatibility than those previously described.

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Encapsulation of a compartmentalized cytoplasm mimic within a lipid membrane by microfluidics

Abstract

All Science Journal Classification (ASJC) codes

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