Encapsulation of Streptococcus uberis: Influence of storage and cultural conditions

K. R. Matthews; B. M. Jayarao; A. J. Guidry; E. F. Erbe; W. P. Wergin; S. P. Oliver

doi:10.1016/0378-1135(94)90171-6

Encapsulation of Streptococcus uberis: Influence of storage and cultural conditions

K. R. Matthews, B. M. Jayarao, A. J. Guidry, E. F. Erbe, W. P. Wergin, S. P. Oliver

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Streptococcus uberis (n = 100) isolated from bovine mammary secretions were assessed by India ink for expression of capsule. Organisms were evaluated under four conditions; (1) after primary culture on blood agar, (2) following 5 passages on blood agar, (3) after 5 passages in Trypticase Soy Broth (TSB), and (4) after storage in 10% skim milk. Strains from primary culture (44 of 100) were positive for an unstained halo (capsule) by the India ink method. Number of strains expressing capsule decreased greatly after passage and following storage. Freeze-etching followed by electron microscopy confirmed results of India ink preparations. Strains were also cultured in various media to determine influence of medium components on capsule expression. Todd-Hewitt medium supplemented with either serum or egg yolk enhanced the size of capsule expressed. Results of this study may aid researchers investigating the pathogenicity of S. uberis.

Original language	English (US)
Pages (from-to)	361-367
Number of pages	7
Journal	Veterinary Microbiology
Volume	39
Issue number	3-4
DOIs	https://doi.org/10.1016/0378-1135(94)90171-6
State	Published - Apr 1994

All Science Journal Classification (ASJC) codes

Microbiology
General Veterinary

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title = "Encapsulation of Streptococcus uberis: Influence of storage and cultural conditions",

abstract = "Streptococcus uberis (n = 100) isolated from bovine mammary secretions were assessed by India ink for expression of capsule. Organisms were evaluated under four conditions; (1) after primary culture on blood agar, (2) following 5 passages on blood agar, (3) after 5 passages in Trypticase Soy Broth (TSB), and (4) after storage in 10% skim milk. Strains from primary culture (44 of 100) were positive for an unstained halo (capsule) by the India ink method. Number of strains expressing capsule decreased greatly after passage and following storage. Freeze-etching followed by electron microscopy confirmed results of India ink preparations. Strains were also cultured in various media to determine influence of medium components on capsule expression. Todd-Hewitt medium supplemented with either serum or egg yolk enhanced the size of capsule expressed. Results of this study may aid researchers investigating the pathogenicity of S. uberis.",

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year = "1994",

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T2 - Influence of storage and cultural conditions

AU - Matthews, K. R.

AU - Jayarao, B. M.

AU - Guidry, A. J.

AU - Erbe, E. F.

AU - Wergin, W. P.

AU - Oliver, S. P.

PY - 1994/4

Y1 - 1994/4

N2 - Streptococcus uberis (n = 100) isolated from bovine mammary secretions were assessed by India ink for expression of capsule. Organisms were evaluated under four conditions; (1) after primary culture on blood agar, (2) following 5 passages on blood agar, (3) after 5 passages in Trypticase Soy Broth (TSB), and (4) after storage in 10% skim milk. Strains from primary culture (44 of 100) were positive for an unstained halo (capsule) by the India ink method. Number of strains expressing capsule decreased greatly after passage and following storage. Freeze-etching followed by electron microscopy confirmed results of India ink preparations. Strains were also cultured in various media to determine influence of medium components on capsule expression. Todd-Hewitt medium supplemented with either serum or egg yolk enhanced the size of capsule expressed. Results of this study may aid researchers investigating the pathogenicity of S. uberis.

AB - Streptococcus uberis (n = 100) isolated from bovine mammary secretions were assessed by India ink for expression of capsule. Organisms were evaluated under four conditions; (1) after primary culture on blood agar, (2) following 5 passages on blood agar, (3) after 5 passages in Trypticase Soy Broth (TSB), and (4) after storage in 10% skim milk. Strains from primary culture (44 of 100) were positive for an unstained halo (capsule) by the India ink method. Number of strains expressing capsule decreased greatly after passage and following storage. Freeze-etching followed by electron microscopy confirmed results of India ink preparations. Strains were also cultured in various media to determine influence of medium components on capsule expression. Todd-Hewitt medium supplemented with either serum or egg yolk enhanced the size of capsule expressed. Results of this study may aid researchers investigating the pathogenicity of S. uberis.

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ER -

Encapsulation of Streptococcus uberis: Influence of storage and cultural conditions

Abstract

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