TY - JOUR
T1 - Engineered Microenvironment for Manufacturing Human Pluripotent Stem Cell-Derived Vascular Smooth Muscle Cells
AU - Lin, Haishuang
AU - Qiu, Xuefeng
AU - Du, Qian
AU - Li, Qiang
AU - Wang, Ou
AU - Akert, Leonard
AU - Wang, Zhanqi
AU - Anderson, Dirk
AU - Liu, Kan
AU - Gu, Linxia
AU - Zhang, Chi
AU - Lei, Yuguo
N1 - Publisher Copyright:
© 2018 The Authors
PY - 2019/1/8
Y1 - 2019/1/8
N2 - Human pluripotent stem cell-derived vascular smooth muscle cells (hPSC-VSMCs) are of great value for disease modeling, drug screening, cell therapies, and tissue engineering. However, producing a high quantity of hPSC-VSMCs with current cell culture technologies remains very challenging. Here, we report a scalable method for manufacturing hPSC-VSMCs in alginate hydrogel microtubes (i.e., AlgTubes), which protect cells from hydrodynamic stresses and limit cell mass to <400 μm to ensure efficient mass transport. The tubes provide cells a friendly microenvironment, leading to extremely high culture efficiency. We have shown that hPSC-VSMCs can be generated in 10 days with high viability, high purity, and high yield (∼5.0 × 10 8 cells/mL). Phenotype and gene expression showed that VSMCs made in AlgTubes and VSMCs made in 2D cultures were similar overall. However, AlgTube-VSMCs had higher expression of genes related to vasculature development and angiogenesis, and 2D-VSMCs had higher expression of genes related to cell death and biosynthetic processes.
AB - Human pluripotent stem cell-derived vascular smooth muscle cells (hPSC-VSMCs) are of great value for disease modeling, drug screening, cell therapies, and tissue engineering. However, producing a high quantity of hPSC-VSMCs with current cell culture technologies remains very challenging. Here, we report a scalable method for manufacturing hPSC-VSMCs in alginate hydrogel microtubes (i.e., AlgTubes), which protect cells from hydrodynamic stresses and limit cell mass to <400 μm to ensure efficient mass transport. The tubes provide cells a friendly microenvironment, leading to extremely high culture efficiency. We have shown that hPSC-VSMCs can be generated in 10 days with high viability, high purity, and high yield (∼5.0 × 10 8 cells/mL). Phenotype and gene expression showed that VSMCs made in AlgTubes and VSMCs made in 2D cultures were similar overall. However, AlgTube-VSMCs had higher expression of genes related to vasculature development and angiogenesis, and 2D-VSMCs had higher expression of genes related to cell death and biosynthetic processes.
UR - https://www.scopus.com/pages/publications/85059828418
UR - https://www.scopus.com/pages/publications/85059828418#tab=citedBy
U2 - 10.1016/j.stemcr.2018.11.009
DO - 10.1016/j.stemcr.2018.11.009
M3 - Article
C2 - 30527760
AN - SCOPUS:85059828418
SN - 2213-6711
VL - 12
SP - 84
EP - 97
JO - Stem Cell Reports
JF - Stem Cell Reports
IS - 1
ER -