Engineering bromodomains with a photoactive amino acid by engaging 'Privileged' tRNA synthetases

Shana Wagner; Babu Sudhamalla; Philip Mannes; Sushma Sappa; Sam Kavoosi; Debasis Dey; Sinan Wang; Kabirul Islam

doi:10.1039/c9cc09891g

Engineering bromodomains with a photoactive amino acid by engaging 'Privileged' tRNA synthetases

Shana Wagner
, Babu Sudhamalla
, Philip Mannes
, Sushma Sappa
, Sam Kavoosi
, Debasis Dey
, Sinan Wang
, Kabirul Islam

Division of Engineering and Science (Abington)

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Site-specific placement of unnatural amino acids, particularly those responsive to light, offers an elegant approach to control protein function and capture their fleeting 'interactome'. Herein, we have resurrected 4-(trifluoromethyldiazirinyl)-phenylalanine, an underutilized photo-crosslinker, by introducing several key features including easy synthetic access, site-specific incorporation by 'privileged' synthetases and superior crosslinking efficiency, to develop photo-crosslinkable bromodomains suitable for 'interactome' profiling.

Original language	English (US)
Pages (from-to)	3641-3644
Number of pages	4
Journal	Chemical Communications
Volume	56
Issue number	25
DOIs	https://doi.org/10.1039/c9cc09891g
State	Published - Mar 28 2020

All Science Journal Classification (ASJC) codes

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c9cc09891g

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abstract = "Site-specific placement of unnatural amino acids, particularly those responsive to light, offers an elegant approach to control protein function and capture their fleeting 'interactome'. Herein, we have resurrected 4-(trifluoromethyldiazirinyl)-phenylalanine, an underutilized photo-crosslinker, by introducing several key features including easy synthetic access, site-specific incorporation by 'privileged' synthetases and superior crosslinking efficiency, to develop photo-crosslinkable bromodomains suitable for 'interactome' profiling.",

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AU - Wagner, Shana

AU - Sudhamalla, Babu

AU - Mannes, Philip

AU - Sappa, Sushma

AU - Kavoosi, Sam

AU - Dey, Debasis

AU - Wang, Sinan

AU - Islam, Kabirul

PY - 2020/3/28

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AB - Site-specific placement of unnatural amino acids, particularly those responsive to light, offers an elegant approach to control protein function and capture their fleeting 'interactome'. Herein, we have resurrected 4-(trifluoromethyldiazirinyl)-phenylalanine, an underutilized photo-crosslinker, by introducing several key features including easy synthetic access, site-specific incorporation by 'privileged' synthetases and superior crosslinking efficiency, to develop photo-crosslinkable bromodomains suitable for 'interactome' profiling.

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UR - https://www.scopus.com/pages/publications/85082542287#tab=citedBy

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ER -

Engineering bromodomains with a photoactive amino acid by engaging 'Privileged' tRNA synthetases

Abstract

All Science Journal Classification (ASJC) codes

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