Engineering Elastic Nano- and Micro-Patterns and Textures for Directed Cell Motility

Erdem D. Tabdanov; Alexander S. Zhovmer; Vikram Puram; Paolo P. Provenzano

doi:10.1016/j.xpro.2019.100013

Engineering Elastic Nano- and Micro-Patterns and Textures for Directed Cell Motility

Erdem D. Tabdanov, Alexander S. Zhovmer, Vikram Puram, Paolo P. Provenzano

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We present a reproducible protocol for fabrication of polyacrylamide (PAA) hydrogel-based nano-patterns and nano-textures with a wide range of elastic rigidities to study fundamental cell behaviors, such as mechanosensitivity and motility. We explore the benefits of this protocol by successfully testing the compatibility of the PAA platforms with super-resolution microscopy, which is largely unavailable with platforms of nano-scale textures made from different polymers. We also utilized soft and rigid nano-textures to study the mechanosensing basis of T cell behavior and phenotype. For complete information on the generation and use of this protocol, please refer to Tabdanov et al. (2018b).

Original language	English (US)
Article number	100013
Journal	STAR Protocols
Volume	1
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2019.100013
State	Published - Jun 19 2020

All Science Journal Classification (ASJC) codes

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2019.100013

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abstract = "We present a reproducible protocol for fabrication of polyacrylamide (PAA) hydrogel-based nano-patterns and nano-textures with a wide range of elastic rigidities to study fundamental cell behaviors, such as mechanosensitivity and motility. We explore the benefits of this protocol by successfully testing the compatibility of the PAA platforms with super-resolution microscopy, which is largely unavailable with platforms of nano-scale textures made from different polymers. We also utilized soft and rigid nano-textures to study the mechanosensing basis of T cell behavior and phenotype. For complete information on the generation and use of this protocol, please refer to Tabdanov et al. (2018b).",

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AB - We present a reproducible protocol for fabrication of polyacrylamide (PAA) hydrogel-based nano-patterns and nano-textures with a wide range of elastic rigidities to study fundamental cell behaviors, such as mechanosensitivity and motility. We explore the benefits of this protocol by successfully testing the compatibility of the PAA platforms with super-resolution microscopy, which is largely unavailable with platforms of nano-scale textures made from different polymers. We also utilized soft and rigid nano-textures to study the mechanosensing basis of T cell behavior and phenotype. For complete information on the generation and use of this protocol, please refer to Tabdanov et al. (2018b).

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Engineering Elastic Nano- and Micro-Patterns and Textures for Directed Cell Motility

Abstract

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