TY - JOUR
T1 - Engineering the mangrove soil microbiome for selection of polyethylene terephthalate-transforming bacterial consortia
AU - Jiménez, Diego Javier
AU - Chaparro, Dayanne
AU - Sierra, Felipe
AU - Custer, Gordon F.
AU - Feuerriegel, Golo
AU - Chuvochina, Maria
AU - Diaz-Garcia, Laura
AU - Mendes, Lucas William
AU - Ortega Santiago, Yina Paola
AU - Rubiano-Labrador, Carolina
AU - Salcedo Galan, Felipe
AU - Streit, Wolfgang R.
AU - Dini-Andreote, Francisco
AU - Reyes, Alejandro
AU - Rosado, Alexandre Soares
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2025/1
Y1 - 2025/1
N2 - Mangroves are impacted by multiple environmental stressors, including sea level rise, erosion, and plastic pollution. Thus, mangrove soil may be an excellent source of as yet unknown plastic-transforming microorganisms. Here, we assess the impact of polyethylene terephthalate (PET) particles and seawater intrusion on the mangrove soil microbiome and report an enrichment culture experiment to artificially select PET-transforming microbial consortia. The analysis of metagenome-assembled genomes of two bacterial consortia revealed that PET catabolism can be performed by multiple taxa, of which particular species harbored putative novel PET-active hydrolases. A key member of these consortia (Mangrovimarina plasticivorans gen. nov., sp. nov.) was found to contain two genes encoding monohydroxyethyl terephthalate hydrolases. This study provides insights into the development of strategies for harnessing soil microbiomes, thereby advancing our understanding of the ecology and enzymology involved in microbial-mediated PET transformations in marine-associated systems.
AB - Mangroves are impacted by multiple environmental stressors, including sea level rise, erosion, and plastic pollution. Thus, mangrove soil may be an excellent source of as yet unknown plastic-transforming microorganisms. Here, we assess the impact of polyethylene terephthalate (PET) particles and seawater intrusion on the mangrove soil microbiome and report an enrichment culture experiment to artificially select PET-transforming microbial consortia. The analysis of metagenome-assembled genomes of two bacterial consortia revealed that PET catabolism can be performed by multiple taxa, of which particular species harbored putative novel PET-active hydrolases. A key member of these consortia (Mangrovimarina plasticivorans gen. nov., sp. nov.) was found to contain two genes encoding monohydroxyethyl terephthalate hydrolases. This study provides insights into the development of strategies for harnessing soil microbiomes, thereby advancing our understanding of the ecology and enzymology involved in microbial-mediated PET transformations in marine-associated systems.
UR - http://www.scopus.com/inward/record.url?scp=85204485969&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85204485969&partnerID=8YFLogxK
U2 - 10.1016/j.tibtech.2024.08.013
DO - 10.1016/j.tibtech.2024.08.013
M3 - Article
C2 - 39304351
AN - SCOPUS:85204485969
SN - 0167-7799
VL - 43
SP - 162
EP - 183
JO - Trends in Biotechnology
JF - Trends in Biotechnology
IS - 1
ER -
