TY - JOUR
T1 - Enhanced human lysozyme production by kluyveromyces lactis
AU - Huang, Eric Lu
AU - Demirci, Ali
N1 - Funding Information:
Acknowledgements The funding for this project was provided by Pennsylvania Agricultural Experimental Station. Authors would like to have special thanks to Ms. Lisa Wilken of Texas A&M University and Dr. Mike Zhang of Virginia Tech on lysozyme analysis.
PY - 2009
Y1 - 2009
N2 - An attempt to enhance recombinant human lysozyme production by Kluyveromyces lactis K7 was performed in this study. In this study, the production of recombinant human lysozyme was investigated using shake flasks and bioreactor under different cultivation conditions. It was demonstrated that 25 °C could enhance human lysozyme production when compared with other temperatures tested. This study also demonstrated that higher biomass did not necessarily produce higher human lysozyme, and it was clear that human lysozyme production was enhanced under unfavorable conditions such as low acidity and oxygen limitation. Cultivation condition with no pH control demonstrated better production than with pH controlled near neutrality. Oxygen limitation also resulted in higher recombinant human lysozyme production. Overall human lysozyme was increased from 64.1 U/ml in flask to 123.6 U/ml in fed-batch fermentation using 4.5% (w/v) glucose initially and fed with concentrated lactose to achieve 9% lactose concentration.
AB - An attempt to enhance recombinant human lysozyme production by Kluyveromyces lactis K7 was performed in this study. In this study, the production of recombinant human lysozyme was investigated using shake flasks and bioreactor under different cultivation conditions. It was demonstrated that 25 °C could enhance human lysozyme production when compared with other temperatures tested. This study also demonstrated that higher biomass did not necessarily produce higher human lysozyme, and it was clear that human lysozyme production was enhanced under unfavorable conditions such as low acidity and oxygen limitation. Cultivation condition with no pH control demonstrated better production than with pH controlled near neutrality. Oxygen limitation also resulted in higher recombinant human lysozyme production. Overall human lysozyme was increased from 64.1 U/ml in flask to 123.6 U/ml in fed-batch fermentation using 4.5% (w/v) glucose initially and fed with concentrated lactose to achieve 9% lactose concentration.
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U2 - 10.1007/s11947-008-0062-1
DO - 10.1007/s11947-008-0062-1
M3 - Article
AN - SCOPUS:65249146586
SN - 1935-5130
VL - 2
SP - 222
EP - 228
JO - Food and Bioprocess Technology
JF - Food and Bioprocess Technology
IS - 2
ER -