TY - JOUR
T1 - Enhanced human lysozyme production in biofilm reactor by Kluyveromyces lactis K7
AU - Ercan, Duygu
AU - Demirci, Ali
N1 - Funding Information:
This work was supported in part by Turkish Ministry of Education by providing scholarship to Duygu Ercan, the Pennsylvania Agricultural Experiment Station , and Agricultural Sciences Graduate Student Competitive Grants Program (the Alex and Jessie C. Black Research Fund) . We also thank Dr. Anthony L. Pometto III for helping manufacturing plastic composite supports and Mr. Ilker Oruc for helping to draw the PCS biofilm reactor design. Moreover, we thank Hilmar Ingredients Inc. (Hilmar, CA) for providing lactose for the study. Finally, the authors confirm that they have no conflict of interest to declare.
Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2014/11/5
Y1 - 2014/11/5
N2 - Lysozyme is an antimicrobial compound, which has been used in pharmaceutical and food industries. Chicken egg is the commercial source of lysozyme. However, human lysozyme is more effective and safer than egg-white lysozyme. Human milk is an important source for human lysozyme, but it is not feasible to provide the needed lysozyme commercially. Biofilm reactors provide passive immobilization of cells onto the solid support, which may lead to higher productivity. The aim was to evaluate the fermentation medium composition for enhanced human lysozyme production by Kluyveromyces lactis K7 in biofilm reactor with plastic composite supports. Yeast nitrogen base was selected as the best nitrogen source when compared to the yeast extract and corn steep liquor. Moreover, inhibition effect of NaCl and NH4Cl at the concentrations of 25 and 50mM was observed. Three factors Box-Behnken response surface design was conducted and the results suggested 16.3% lactose, 1.2% casamino acid, 0.8% yeast nitrogen base as optimum medium composition for maximum human lysozyme production. Overall, the human lysozyme production by K. lactis K7 was increased to 173U/ml, which is about 23% improvement in biofilm reactor and 57% improvement compared to the suspended-cell fermentation.
AB - Lysozyme is an antimicrobial compound, which has been used in pharmaceutical and food industries. Chicken egg is the commercial source of lysozyme. However, human lysozyme is more effective and safer than egg-white lysozyme. Human milk is an important source for human lysozyme, but it is not feasible to provide the needed lysozyme commercially. Biofilm reactors provide passive immobilization of cells onto the solid support, which may lead to higher productivity. The aim was to evaluate the fermentation medium composition for enhanced human lysozyme production by Kluyveromyces lactis K7 in biofilm reactor with plastic composite supports. Yeast nitrogen base was selected as the best nitrogen source when compared to the yeast extract and corn steep liquor. Moreover, inhibition effect of NaCl and NH4Cl at the concentrations of 25 and 50mM was observed. Three factors Box-Behnken response surface design was conducted and the results suggested 16.3% lactose, 1.2% casamino acid, 0.8% yeast nitrogen base as optimum medium composition for maximum human lysozyme production. Overall, the human lysozyme production by K. lactis K7 was increased to 173U/ml, which is about 23% improvement in biofilm reactor and 57% improvement compared to the suspended-cell fermentation.
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U2 - 10.1016/j.bej.2014.04.013
DO - 10.1016/j.bej.2014.04.013
M3 - Article
AN - SCOPUS:84908384784
SN - 1369-703X
VL - 92
SP - 2
EP - 8
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
ER -