TY - GEN
T1 - Enhanced pullulan production in a biofilm reactor by using response surface methodology
AU - Cheng, Kuan Chen
AU - Demirci, Ali
AU - Catchmark, Jeffrey M.
PY - 2010
Y1 - 2010
N2 - In this study, response surface methodology using Box-Behnken design was employed to study the effects of sucrose and nitrogen concentrations on pullulan production. Total of 15 experimental runs were carried out in a plastic composite support biofilm reactor. Three-dimensional response surface was generated to evaluate the effects of the factors and to obtain the optimum condition of each factor for maximum pullulan production. After 7-day fermentation with optimum condition, the pullulan production reached 60.7 g/L, which was 1.8 times higher than the result from original medium, and was the highest yield reported to date. The quality analysis demonstrated that the purity of produced pullulan was 95.2% and its viscosity was 2.5 centipoise (cP). Fourier Transform Infrared Spectroscopy (FTIR) also suggested that the produced exopolysaccharide was pullulan. Overall, this study demonstrated that the response surface methodology can be successfully applied to optimize medium composition for pullulan production in a biofilm reactor and maintained its high purity.
AB - In this study, response surface methodology using Box-Behnken design was employed to study the effects of sucrose and nitrogen concentrations on pullulan production. Total of 15 experimental runs were carried out in a plastic composite support biofilm reactor. Three-dimensional response surface was generated to evaluate the effects of the factors and to obtain the optimum condition of each factor for maximum pullulan production. After 7-day fermentation with optimum condition, the pullulan production reached 60.7 g/L, which was 1.8 times higher than the result from original medium, and was the highest yield reported to date. The quality analysis demonstrated that the purity of produced pullulan was 95.2% and its viscosity was 2.5 centipoise (cP). Fourier Transform Infrared Spectroscopy (FTIR) also suggested that the produced exopolysaccharide was pullulan. Overall, this study demonstrated that the response surface methodology can be successfully applied to optimize medium composition for pullulan production in a biofilm reactor and maintained its high purity.
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M3 - Conference contribution
AN - SCOPUS:78649718992
SN - 9781617388354
T3 - American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010
SP - 1597
EP - 1610
BT - American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010
PB - American Society of Agricultural and Biological Engineers
ER -