Enhanced recombination between λp lac5 and mini-F-lac: The tra regulon is required for recombination enhancement

H. Steven Seifert; Ronald D. Porter

doi:10.1007/BF00330679

Enhanced recombination between λp lac5 and mini-F-lac: The tra regulon is required for recombination enhancement

H. Steven Seifert, Ronald D. Porter

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

F42 lac recombination with λp lac is normally 20-fold to 50-fold higher than recombination between λp lac and a chromosomal lac gene. Transductional crosses with λp lac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42 lac.

Original language	English (US)
Pages (from-to)	269-274
Number of pages	6
Journal	MGG Molecular & General Genetics
Volume	193
Issue number	2
DOIs	https://doi.org/10.1007/BF00330679
State	Published - Feb 1984

All Science Journal Classification (ASJC) codes

Genetics

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title = "Enhanced recombination between λp lac5 and mini-F-lac: The tra regulon is required for recombination enhancement",

abstract = "F42 lac recombination with λp lac is normally 20-fold to 50-fold higher than recombination between λp lac and a chromosomal lac gene. Transductional crosses with λp lac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42 lac.",

author = "Seifert, {H. Steven} and Porter, {Ronald D.}",

year = "1984",

month = feb,

doi = "10.1007/BF00330679",

language = "English (US)",

volume = "193",

pages = "269--274",

journal = "MGG Molecular & General Genetics",

issn = "0026-8925",

publisher = "Springer Science and Business Media Deutschland GmbH",

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TY - JOUR

T1 - Enhanced recombination between λp lac5 and mini-F-lac

T2 - The tra regulon is required for recombination enhancement

AU - Seifert, H. Steven

AU - Porter, Ronald D.

PY - 1984/2

Y1 - 1984/2

N2 - F42 lac recombination with λp lac is normally 20-fold to 50-fold higher than recombination between λp lac and a chromosomal lac gene. Transductional crosses with λp lac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42 lac.

AB - F42 lac recombination with λp lac is normally 20-fold to 50-fold higher than recombination between λp lac and a chromosomal lac gene. Transductional crosses with λp lac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42 lac.

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U2 - 10.1007/BF00330679

DO - 10.1007/BF00330679

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AN - SCOPUS:0021307495

SN - 0026-8925

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SP - 269

EP - 274

JO - MGG Molecular & General Genetics

JF - MGG Molecular & General Genetics

IS - 2

ER -

Enhanced recombination between λp lac5 and mini-F-lac: The tra regulon is required for recombination enhancement

Abstract

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