Abstract
F42 lac recombination with λp lac is normally 20-fold to 50-fold higher than recombination between λp lac and a chromosomal lac gene. Transductional crosses with λp lac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42 lac.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 269-274 |
| Number of pages | 6 |
| Journal | MGG Molecular & General Genetics |
| Volume | 193 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1984 |
All Science Journal Classification (ASJC) codes
- Genetics
Fingerprint
Dive into the research topics of 'Enhanced recombination between λp lac5 and mini-F-lac: The tra regulon is required for recombination enhancement'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver