TY - JOUR
T1 - Enrichment of acid-tolerant sulfide-producing microbes from an acidic pit lake
AU - Liu, Yutong
AU - Macalady, Jennifer L.
AU - Sánchez-España, Javier
AU - Burgos, William D.
N1 - Publisher Copyright:
Copyright © 2024 Liu, Macalady, Sánchez-España and Burgos.
PY - 2024
Y1 - 2024
N2 - High concentrations of harmful metal(loid)s and extreme acidity are persistent environmental concerns in acidic pit lakes. In this study, we examine Cueva de la Mora (CM), a meromictic pit lake in the Iberian Pyrite Belt, Spain, as a model system. Our research aims to explore potential bioremediation strategies to mitigate the impacts of metal(loid)s and acidity in such environments. The major strategy applied in this research is to biologically stimulate sulfate reduction (i.e., biosulfidogenesis) in the deep layer of the lake to promote the formation of low-solubility sulfide minerals. Previous omics-based studies of CM have shown that several sulfate-reducing bacteria (SRB) taxa are present in the deep layer. However, their activities are likely limited by the availability of electron donors for sulfide production. Therefore, different amendments (glycerol, elemental sulfur, and glycerol + elemental sulfur) were tested to promote sulfide production and enrich acid-tolerant sulfide-producing microbes. Our results showed that glycerol stimulated dissimilatory sulfate reduction much faster than elemental sulfur alone, suggesting that electron donor limitations control sulfide production. Furthermore, the combined addition of glycerol and elemental sulfur (S(0)) resulted in the highest level of sulfide production. This indicates that S(0) can play a significant role as an electron acceptor in further promoting sulfide production when a suitable electron donor is present. Microbial community analysis revealed that Desulfosporosinus acididurans, a previously discovered acid-tolerant SRB, was enriched and became the dominant species in incubations with glycerol only (~76–96% abundance) or the combination of glycerol and S(0) (~93–99% abundance).
AB - High concentrations of harmful metal(loid)s and extreme acidity are persistent environmental concerns in acidic pit lakes. In this study, we examine Cueva de la Mora (CM), a meromictic pit lake in the Iberian Pyrite Belt, Spain, as a model system. Our research aims to explore potential bioremediation strategies to mitigate the impacts of metal(loid)s and acidity in such environments. The major strategy applied in this research is to biologically stimulate sulfate reduction (i.e., biosulfidogenesis) in the deep layer of the lake to promote the formation of low-solubility sulfide minerals. Previous omics-based studies of CM have shown that several sulfate-reducing bacteria (SRB) taxa are present in the deep layer. However, their activities are likely limited by the availability of electron donors for sulfide production. Therefore, different amendments (glycerol, elemental sulfur, and glycerol + elemental sulfur) were tested to promote sulfide production and enrich acid-tolerant sulfide-producing microbes. Our results showed that glycerol stimulated dissimilatory sulfate reduction much faster than elemental sulfur alone, suggesting that electron donor limitations control sulfide production. Furthermore, the combined addition of glycerol and elemental sulfur (S(0)) resulted in the highest level of sulfide production. This indicates that S(0) can play a significant role as an electron acceptor in further promoting sulfide production when a suitable electron donor is present. Microbial community analysis revealed that Desulfosporosinus acididurans, a previously discovered acid-tolerant SRB, was enriched and became the dominant species in incubations with glycerol only (~76–96% abundance) or the combination of glycerol and S(0) (~93–99% abundance).
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U2 - 10.3389/fmicb.2024.1475137
DO - 10.3389/fmicb.2024.1475137
M3 - Article
C2 - 39539707
AN - SCOPUS:85208991236
SN - 1664-302X
VL - 15
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 1475137
ER -