Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice

Yuan Wan; Mackenzie Maurer; Hong Zhang He; Yi Qiu Xia; Wen Long Zhang; Si Jie Hao; Nelson S. Yee; Si Yang Zheng

doi:10.1109/TRANSDUCERS.2019.8808761

Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice

Yuan Wan, Mackenzie Maurer, Hong Zhang He, Yi Qiu Xia, Wen Long Zhang, Si Jie Hao, Nelson S. Yee, Si Yang Zheng

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Extracellular vesicles (EVs), from clinical plasma samples, have recently been used towards applications of cancer diagnostics and treatment monitoring. However, clinical translation has been limited by technical challenges in EV enrichment. Thus, we have reported on the development of a microdevice platform that utilizes dual capture principles for improved EV isolation and enrichment. By utilizing (1) an EV-specific lipid nanoprobe-grafted silica for isolation via EV tethering and (2) EV size-matched nanostructures for isolation via physical trapping, isolation efficiency is increased by nearly three times. Furthermore, this continuous-flow microdevice eliminates plasma protein contaminants by upwards of ~97%.

Original language	English (US)
Title of host publication	2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	273-276
Number of pages	4
ISBN (Electronic)	9781728120072
DOIs	https://doi.org/10.1109/TRANSDUCERS.2019.8808761
State	Published - Jun 2019
Event	20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany Duration: Jun 23 2019 → Jun 27 2019

Publication series

Name	2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Conference

Conference	20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
Country/Territory	Germany
City	Berlin
Period	6/23/19 → 6/27/19

All Science Journal Classification (ASJC) codes

Process Chemistry and Technology
Spectroscopy
Electrical and Electronic Engineering
Mechanical Engineering
Electronic, Optical and Magnetic Materials
Control and Optimization
Instrumentation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/TRANSDUCERS.2019.8808761

Cite this

Wan, Y., Maurer, M., He, H. Z., Xia, Y. Q., Zhang, W. L., Hao, S. J., Yee, N. S., & Zheng, S. Y. (2019). Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII (pp. 273-276). Article 8808761 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2019.8808761

Wan, Yuan ; Maurer, Mackenzie ; He, Hong Zhang et al. / Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice. 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 273-276 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

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title = "Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice",

abstract = "Extracellular vesicles (EVs), from clinical plasma samples, have recently been used towards applications of cancer diagnostics and treatment monitoring. However, clinical translation has been limited by technical challenges in EV enrichment. Thus, we have reported on the development of a microdevice platform that utilizes dual capture principles for improved EV isolation and enrichment. By utilizing (1) an EV-specific lipid nanoprobe-grafted silica for isolation via EV tethering and (2) EV size-matched nanostructures for isolation via physical trapping, isolation efficiency is increased by nearly three times. Furthermore, this continuous-flow microdevice eliminates plasma protein contaminants by upwards of ~97%.",

author = "Yuan Wan and Mackenzie Maurer and He, {Hong Zhang} and Xia, {Yi Qiu} and Zhang, {Wen Long} and Hao, {Si Jie} and Yee, {Nelson S.} and Zheng, {Si Yang}",

year = "2019",

month = jun,

doi = "10.1109/TRANSDUCERS.2019.8808761",

language = "English (US)",

series = "2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "273--276",

booktitle = "2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII",

address = "United States",

note = "20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII ; Conference date: 23-06-2019 Through 27-06-2019",

}

Wan, Y, Maurer, M, He, HZ, Xia, YQ, Zhang, WL, Hao, SJ, Yee, NS & Zheng, SY 2019, Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice. in 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII., 8808761, 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Institute of Electrical and Electronics Engineers Inc., pp. 273-276, 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Berlin, Germany, 6/23/19. https://doi.org/10.1109/TRANSDUCERS.2019.8808761

Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice. / Wan, Yuan; Maurer, Mackenzie; He, Hong Zhang et al.
2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. p. 273-276 8808761 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice

AU - Wan, Yuan

AU - Maurer, Mackenzie

AU - He, Hong Zhang

AU - Xia, Yi Qiu

AU - Zhang, Wen Long

AU - Hao, Si Jie

AU - Yee, Nelson S.

AU - Zheng, Si Yang

PY - 2019/6

Y1 - 2019/6

N2 - Extracellular vesicles (EVs), from clinical plasma samples, have recently been used towards applications of cancer diagnostics and treatment monitoring. However, clinical translation has been limited by technical challenges in EV enrichment. Thus, we have reported on the development of a microdevice platform that utilizes dual capture principles for improved EV isolation and enrichment. By utilizing (1) an EV-specific lipid nanoprobe-grafted silica for isolation via EV tethering and (2) EV size-matched nanostructures for isolation via physical trapping, isolation efficiency is increased by nearly three times. Furthermore, this continuous-flow microdevice eliminates plasma protein contaminants by upwards of ~97%.

AB - Extracellular vesicles (EVs), from clinical plasma samples, have recently been used towards applications of cancer diagnostics and treatment monitoring. However, clinical translation has been limited by technical challenges in EV enrichment. Thus, we have reported on the development of a microdevice platform that utilizes dual capture principles for improved EV isolation and enrichment. By utilizing (1) an EV-specific lipid nanoprobe-grafted silica for isolation via EV tethering and (2) EV size-matched nanostructures for isolation via physical trapping, isolation efficiency is increased by nearly three times. Furthermore, this continuous-flow microdevice eliminates plasma protein contaminants by upwards of ~97%.

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M3 - Conference contribution

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SP - 273

EP - 276

BT - 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Y2 - 23 June 2019 through 27 June 2019

ER -

Wan Y, Maurer M, He HZ, Xia YQ, Zhang WL, Hao SJ et al. Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc. 2019. p. 273-276. 8808761. (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). doi: 10.1109/TRANSDUCERS.2019.8808761

Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice

Abstract

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All Science Journal Classification (ASJC) codes

UN SDGs

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