Enumeration of Fluorescent Pseudomonads on Poultry by using the Hydrophobic‐Grid Membrane Filter Method

Accurate enumeration of fluorescent pseudomonads on poultry was possible by using hydrophobic‐grid membrane filters and S‐I agar. In methionine‐cysteine broth, fluorescent isolates produced more volatile sulfides and yielded higher odor scores than nonfluorescent isolates. Fluorescent pigment had an inhibitory effect on the growth of nonfluorescent isolates. Resuscitation on Trypticase Soy Agar resulted in a significant increase in the recovery of fluorescent pseudomonads. Shelf life was negatively correlated (r = ‐0.86) with initial numbers of fluorescent pseudomonads.