Environment determines fidelity for an RNA virus replicase

Justin S. Pita, Joachim R. De Miranda, William L. Schneider, Marilyn J. Roossinck

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


The rate of insertion and deletion mutations of the replicase of Cucumber mosaic virus (CMV) was determined in planta by using a parasitic satellite RNA (satRNA) as a reporter. We found that the CMV replicase had different fidelity in different environments, with important implications in viral disease evolution. Insertions were very rare events, irrespective of the region of the satRNA genome assayed and independent of the hosts tested. On the other hand, deletion events were more frequent but were restricted to a highly structured region of the reporter. Deletion mutation rates were different for the two hosts tested, although the mutation distribution was not influenced by the hosts. Moreover, hot spots with high mutation rates were identified on the satRNA genome.

Original languageEnglish (US)
Pages (from-to)9072-9077
Number of pages6
JournalJournal of virology
Issue number17
StatePublished - Sep 2007

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology


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