TY - CHAP
T1 - Enzyme-gold nanoparticle bioconjugates
T2 - Quantification of particle stoichiometry and enzyme specific activity
AU - Keighron, Jacqueline D.
AU - Keating, Christine D.
PY - 2013
Y1 - 2013
N2 - Enzyme-gold nanoparticle bioconjugates have a wide variety of uses ranging from nanoreactors to sensors and model systems. While easy to make, these bioconjugates are often not well characterized. This protocol outlines preparation of enzyme-nanoparticle bioconjugates and two complementary methods for quantifying enzyme:nanoparticle stoichiometry from which the specific activity of the adsorbed enzymes can be determined. Characterizations such as these can aid researchers in improving the design and application of future bioconjugate systems.
AB - Enzyme-gold nanoparticle bioconjugates have a wide variety of uses ranging from nanoreactors to sensors and model systems. While easy to make, these bioconjugates are often not well characterized. This protocol outlines preparation of enzyme-nanoparticle bioconjugates and two complementary methods for quantifying enzyme:nanoparticle stoichiometry from which the specific activity of the adsorbed enzymes can be determined. Characterizations such as these can aid researchers in improving the design and application of future bioconjugate systems.
UR - http://www.scopus.com/inward/record.url?scp=84883183845&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883183845&partnerID=8YFLogxK
U2 - 10.1007/978-1-62703-468-5_13
DO - 10.1007/978-1-62703-468-5_13
M3 - Chapter
C2 - 23749577
AN - SCOPUS:84883183845
SN - 9781627034678
T3 - Methods in Molecular Biology
SP - 163
EP - 174
BT - NanoBiotechnology Protocols
PB - Humana Press Inc.
ER -