TY - JOUR
T1 - Epidermal antigen preservation with freezing
AU - Helm, Klaus
AU - Lane, A. T.
AU - Muhlbauer, J. E.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1986
Y1 - 1986
N2 - Antigen preservation in human foreskin was evaluated after prolonged storage. Foreskin sections previously separated and stored were examined after 24 h, 6 days, 102 days, 364 days, and 493 days, using indirect immunofluorescence techniques. Each foreskin was evaluated with high-titer human antisera against bullous pemphigoid and pemphigus antigens, rabbit antiserum against laminin, and IgG murine monoclonal antibodies against OKT6, OKIa, AF-1, and Kab-3. Each section was studied and scored by three independent observers. Except for decreased fluorescent staining of pemphigus antigens, no decrease of the immunofluorescence titers was seen over the period of observation.
AB - Antigen preservation in human foreskin was evaluated after prolonged storage. Foreskin sections previously separated and stored were examined after 24 h, 6 days, 102 days, 364 days, and 493 days, using indirect immunofluorescence techniques. Each foreskin was evaluated with high-titer human antisera against bullous pemphigoid and pemphigus antigens, rabbit antiserum against laminin, and IgG murine monoclonal antibodies against OKT6, OKIa, AF-1, and Kab-3. Each section was studied and scored by three independent observers. Except for decreased fluorescent staining of pemphigus antigens, no decrease of the immunofluorescence titers was seen over the period of observation.
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U2 - 10.1097/00000372-198612000-00006
DO - 10.1097/00000372-198612000-00006
M3 - Article
C2 - 3544901
AN - SCOPUS:0022868094
SN - 0193-1091
VL - 8
SP - 492
EP - 495
JO - American Journal of Dermatopathology
JF - American Journal of Dermatopathology
IS - 6
ER -