Error in the calibration of the mgatp chemical‐shift limit: Effects on the determination of free magnesium by 31P NMR spectroscopy

Timothy Mosher; Gerald D. Williams; Chris Doumen; Kathryn F. Lanoue; Michael B. Smith

doi:10.1002/mrm.1910240117

Error in the calibration of the mgatp chemical‐shift limit: Effects on the determination of free magnesium by ³¹P NMR spectroscopy

Timothy Mosher, Gerald D. Williams, Chris Doumen, Kathryn F. Lanoue, Michael B. Smith

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

The measurement of free intracellular magnesium (Mg²⁺) using the ³¹P chemical shifts of ATP requires the use of appropriate calibration solutions to determine the chemical‐shift limits Δ^ATP_αβ and Δ^MgATP_αβ. Solutions containing excess Mg²⁺ contain significant amounts of Mg₂ATP and yield positive errors in the value of Δ^MgATP_αβ. For physiological applications this may overestimate free intracellular Mg²⁺ by as much as 300%. This error may be minimized if appropriate mole ratios of Mg²⁺/ATP are used to calibrate Δ^MgATP_αβ. © 1992 Academic Press, Inc.

Original language	English (US)
Pages (from-to)	163-169
Number of pages	7
Journal	Magnetic Resonance in Medicine
Volume	24
Issue number	1
DOIs	https://doi.org/10.1002/mrm.1910240117
State	Published - Mar 1992

All Science Journal Classification (ASJC) codes

Radiology Nuclear Medicine and imaging

Access to Document

10.1002/mrm.1910240117

Cite this

@article{410de150b91c46469cca8d8f8a9b4c46,

title = "Error in the calibration of the mgatp chemical‐shift limit: Effects on the determination of free magnesium by 31P NMR spectroscopy",

abstract = "The measurement of free intracellular magnesium (Mg2+) using the 31P chemical shifts of ATP requires the use of appropriate calibration solutions to determine the chemical‐shift limits ΔATPαβ and ΔMgATPαβ. Solutions containing excess Mg2+ contain significant amounts of Mg2ATP and yield positive errors in the value of ΔMgATPαβ. For physiological applications this may overestimate free intracellular Mg2+ by as much as 300%. This error may be minimized if appropriate mole ratios of Mg2+/ATP are used to calibrate ΔMgATPαβ. {\textcopyright} 1992 Academic Press, Inc.",

author = "Timothy Mosher and Williams, {Gerald D.} and Chris Doumen and Lanoue, {Kathryn F.} and Smith, {Michael B.}",

year = "1992",

month = mar,

doi = "10.1002/mrm.1910240117",

language = "English (US)",

volume = "24",

pages = "163--169",

journal = "Magnetic Resonance in Medicine",

issn = "0740-3194",

publisher = "John Wiley and Sons Inc.",

number = "1",

}

TY - JOUR

T1 - Error in the calibration of the mgatp chemical‐shift limit

T2 - Effects on the determination of free magnesium by 31P NMR spectroscopy

AU - Mosher, Timothy

AU - Williams, Gerald D.

AU - Doumen, Chris

AU - Lanoue, Kathryn F.

AU - Smith, Michael B.

PY - 1992/3

Y1 - 1992/3

N2 - The measurement of free intracellular magnesium (Mg2+) using the 31P chemical shifts of ATP requires the use of appropriate calibration solutions to determine the chemical‐shift limits ΔATPαβ and ΔMgATPαβ. Solutions containing excess Mg2+ contain significant amounts of Mg2ATP and yield positive errors in the value of ΔMgATPαβ. For physiological applications this may overestimate free intracellular Mg2+ by as much as 300%. This error may be minimized if appropriate mole ratios of Mg2+/ATP are used to calibrate ΔMgATPαβ. © 1992 Academic Press, Inc.

AB - The measurement of free intracellular magnesium (Mg2+) using the 31P chemical shifts of ATP requires the use of appropriate calibration solutions to determine the chemical‐shift limits ΔATPαβ and ΔMgATPαβ. Solutions containing excess Mg2+ contain significant amounts of Mg2ATP and yield positive errors in the value of ΔMgATPαβ. For physiological applications this may overestimate free intracellular Mg2+ by as much as 300%. This error may be minimized if appropriate mole ratios of Mg2+/ATP are used to calibrate ΔMgATPαβ. © 1992 Academic Press, Inc.

UR - http://www.scopus.com/inward/record.url?scp=0026562977&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026562977&partnerID=8YFLogxK

U2 - 10.1002/mrm.1910240117

DO - 10.1002/mrm.1910240117

M3 - Article

C2 - 1556922

AN - SCOPUS:0026562977

SN - 0740-3194

VL - 24

SP - 163

EP - 169

JO - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

IS - 1

ER -

Error in the calibration of the mgatp chemical‐shift limit: Effects on the determination of free magnesium by ³¹P NMR spectroscopy

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this