Error in the calibration of the mgatp chemical‐shift limit: Effects on the determination of free magnesium by 31P NMR spectroscopy

Timothy Mosher; Gerald D. Williams; Chris Doumen; Kathryn F. Lanoue; Michael B. Smith

doi:10.1002/mrm.1910240117

Error in the calibration of the mgatp chemical‐shift limit: Effects on the determination of free magnesium by ³¹P NMR spectroscopy

Timothy Mosher
, Gerald D. Williams
, Chris Doumen
, Kathryn F. Lanoue
, Michael B. Smith

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

The measurement of free intracellular magnesium (Mg²⁺) using the ³¹P chemical shifts of ATP requires the use of appropriate calibration solutions to determine the chemical‐shift limits Δ^ATP_αβ and Δ^MgATP_αβ. Solutions containing excess Mg²⁺ contain significant amounts of Mg₂ATP and yield positive errors in the value of Δ^MgATP_αβ. For physiological applications this may overestimate free intracellular Mg²⁺ by as much as 300%. This error may be minimized if appropriate mole ratios of Mg²⁺/ATP are used to calibrate Δ^MgATP_αβ. © 1992 Academic Press, Inc.

Original language	English (US)
Pages (from-to)	163-169
Number of pages	7
Journal	Magnetic Resonance in Medicine
Volume	24
Issue number	1
DOIs	https://doi.org/10.1002/mrm.1910240117
State	Published - Mar 1992

All Science Journal Classification (ASJC) codes

Radiology Nuclear Medicine and imaging

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10.1002/mrm.1910240117

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title = "Error in the calibration of the mgatp chemical‐shift limit: Effects on the determination of free magnesium by 31P NMR spectroscopy",

abstract = "The measurement of free intracellular magnesium (Mg2+) using the 31P chemical shifts of ATP requires the use of appropriate calibration solutions to determine the chemical‐shift limits ΔATPαβ and ΔMgATPαβ. Solutions containing excess Mg2+ contain significant amounts of Mg2ATP and yield positive errors in the value of ΔMgATPαβ. For physiological applications this may overestimate free intracellular Mg2+ by as much as 300\%. This error may be minimized if appropriate mole ratios of Mg2+/ATP are used to calibrate ΔMgATPαβ. {\textcopyright} 1992 Academic Press, Inc.",

author = "Timothy Mosher and Williams, \{Gerald D.\} and Chris Doumen and Lanoue, \{Kathryn F.\} and Smith, \{Michael B.\}",

year = "1992",

month = mar,

doi = "10.1002/mrm.1910240117",

language = "English (US)",

volume = "24",

pages = "163--169",

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T2 - Effects on the determination of free magnesium by 31P NMR spectroscopy

AU - Mosher, Timothy

AU - Williams, Gerald D.

AU - Doumen, Chris

AU - Lanoue, Kathryn F.

AU - Smith, Michael B.

PY - 1992/3

Y1 - 1992/3

N2 - The measurement of free intracellular magnesium (Mg2+) using the 31P chemical shifts of ATP requires the use of appropriate calibration solutions to determine the chemical‐shift limits ΔATPαβ and ΔMgATPαβ. Solutions containing excess Mg2+ contain significant amounts of Mg2ATP and yield positive errors in the value of ΔMgATPαβ. For physiological applications this may overestimate free intracellular Mg2+ by as much as 300%. This error may be minimized if appropriate mole ratios of Mg2+/ATP are used to calibrate ΔMgATPαβ. © 1992 Academic Press, Inc.

AB - The measurement of free intracellular magnesium (Mg2+) using the 31P chemical shifts of ATP requires the use of appropriate calibration solutions to determine the chemical‐shift limits ΔATPαβ and ΔMgATPαβ. Solutions containing excess Mg2+ contain significant amounts of Mg2ATP and yield positive errors in the value of ΔMgATPαβ. For physiological applications this may overestimate free intracellular Mg2+ by as much as 300%. This error may be minimized if appropriate mole ratios of Mg2+/ATP are used to calibrate ΔMgATPαβ. © 1992 Academic Press, Inc.

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VL - 24

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JO - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

IS - 1

ER -

Error in the calibration of the mgatp chemical‐shift limit: Effects on the determination of free magnesium by ³¹P NMR spectroscopy

Abstract

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