Escherichia coli hydrogenase activity and H2 production under glycerol fermentation at a low pH

Karen Trchounian; Viviana Sanchez-Torres; Thomas K. Wood; Armen Trchounian

doi:10.1016/j.ijhydene.2010.12.128

Escherichia coli hydrogenase activity and H₂ production under glycerol fermentation at a low pH

Karen Trchounian, Viviana Sanchez-Torres, Thomas K. Wood, Armen Trchounian

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

Hydrogenase (Hyd) activity and H₂ production by Escherichia coli were studied at a low pH. H₂ production at pH 5.5 under glycerol fermentation was shown to be ∼1.5-fold higher than that at pH 6.5 or above but less than that under glucose fermentation. It was inhibited by N,N′-dicyclohexylcarbodiimide: H₂ production inhibition was increased with decreasing pH and almost maximal inhibition was observed at pH 5.5. The data on H₂ production by single and double mutants with defects in different Hyd-enzymes and in fhlA gene suggest that under glycerol fermentation at a low pH, Hyd-1, Hyd-2 and Hyd-4 were operating in a reversed, non-H₂ producing mode. Moreover, a role of fhlA gene in Hyd-3 and Hyd-4 activity in H₂ production is proposed under glucose fermentation at a low pH.

Original language	English (US)
Pages (from-to)	4323-4331
Number of pages	9
Journal	International Journal of Hydrogen Energy
Volume	36
Issue number	7
DOIs	https://doi.org/10.1016/j.ijhydene.2010.12.128
State	Published - Apr 2011

All Science Journal Classification (ASJC) codes

Renewable Energy, Sustainability and the Environment
Fuel Technology
Condensed Matter Physics
Energy Engineering and Power Technology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijhydene.2010.12.128

Cite this

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title = "Escherichia coli hydrogenase activity and H2 production under glycerol fermentation at a low pH",

abstract = "Hydrogenase (Hyd) activity and H2 production by Escherichia coli were studied at a low pH. H2 production at pH 5.5 under glycerol fermentation was shown to be ∼1.5-fold higher than that at pH 6.5 or above but less than that under glucose fermentation. It was inhibited by N,N′-dicyclohexylcarbodiimide: H2 production inhibition was increased with decreasing pH and almost maximal inhibition was observed at pH 5.5. The data on H2 production by single and double mutants with defects in different Hyd-enzymes and in fhlA gene suggest that under glycerol fermentation at a low pH, Hyd-1, Hyd-2 and Hyd-4 were operating in a reversed, non-H2 producing mode. Moreover, a role of fhlA gene in Hyd-3 and Hyd-4 activity in H2 production is proposed under glucose fermentation at a low pH.",

author = "Karen Trchounian and Viviana Sanchez-Torres and Wood, {Thomas K.} and Armen Trchounian",

note = "Funding Information: Thanks to Dr. A. Poladyan and A. Pogosyan for help in some measurements. The study was supported by the Grant # 1012-2008 from the Ministry of Education and Science of the Republic of Armenia .",

year = "2011",

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T1 - Escherichia coli hydrogenase activity and H2 production under glycerol fermentation at a low pH

AU - Trchounian, Karen

AU - Sanchez-Torres, Viviana

AU - Wood, Thomas K.

AU - Trchounian, Armen

N1 - Funding Information: Thanks to Dr. A. Poladyan and A. Pogosyan for help in some measurements. The study was supported by the Grant # 1012-2008 from the Ministry of Education and Science of the Republic of Armenia .

PY - 2011/4

Y1 - 2011/4

N2 - Hydrogenase (Hyd) activity and H2 production by Escherichia coli were studied at a low pH. H2 production at pH 5.5 under glycerol fermentation was shown to be ∼1.5-fold higher than that at pH 6.5 or above but less than that under glucose fermentation. It was inhibited by N,N′-dicyclohexylcarbodiimide: H2 production inhibition was increased with decreasing pH and almost maximal inhibition was observed at pH 5.5. The data on H2 production by single and double mutants with defects in different Hyd-enzymes and in fhlA gene suggest that under glycerol fermentation at a low pH, Hyd-1, Hyd-2 and Hyd-4 were operating in a reversed, non-H2 producing mode. Moreover, a role of fhlA gene in Hyd-3 and Hyd-4 activity in H2 production is proposed under glucose fermentation at a low pH.

AB - Hydrogenase (Hyd) activity and H2 production by Escherichia coli were studied at a low pH. H2 production at pH 5.5 under glycerol fermentation was shown to be ∼1.5-fold higher than that at pH 6.5 or above but less than that under glucose fermentation. It was inhibited by N,N′-dicyclohexylcarbodiimide: H2 production inhibition was increased with decreasing pH and almost maximal inhibition was observed at pH 5.5. The data on H2 production by single and double mutants with defects in different Hyd-enzymes and in fhlA gene suggest that under glycerol fermentation at a low pH, Hyd-1, Hyd-2 and Hyd-4 were operating in a reversed, non-H2 producing mode. Moreover, a role of fhlA gene in Hyd-3 and Hyd-4 activity in H2 production is proposed under glucose fermentation at a low pH.

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